Genetic and Physiological Activation of Osmosensitive Gene Expression Mimics Transcriptional Signatures of Pathogen Infection in C. elegans 英文参考文献.docVIP

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Genetic and Physiological Activation of Osmosensitive Gene Expression Mimics Transcriptional Signatures of Pathogen Infection in C. elegans 英文参考文献.doc

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Genetic and Physiological Activation of Osmosensitive Gene Expression Mimics Transcriptional Signatures of Pathogen Infection in C. elegans 英文参考文献

GeneticandPhysiologicalActivationofOsmosensitive GeneExpressionMimicsTranscriptionalSignaturesof PathogenInfectioninC.elegans Anne-KatrinRohlfing1,YanaMiteva1,SridharHannenhalli2,ToddLamitina1* 1DepartmentofPhysiology,UniversityofPennsylvania,Philadelphia,Pennsylvania,UnitedStatesofAmerica,2DepartmentofGenetics,PennCenterforBioinformatics, UniversityofPennsylvania,Philadelphia,Pennsylvania,UnitedStatesofAmerica Abstract The soil-dwelling nematode C. elegans is a powerful system for comparative molecular analyses of environmental stress responsemechanisms.Infectionofwormswithbacterialandfungalpathogenscausestheactivationofwell-characterized innate immune transcriptional programs in pathogen-exposed hypodermal and intestinal tissues. However, the pathophysiological events that drive such transcriptional responses are not understood. Here, we show that infection- activated transcriptional responses are, in large part, recapitulated by either physiological or genetic activation of the osmoticstressresponse.Microarrayprofilingofwildtypewormsexposedtonon-lethalhypertonicityidentifiedasuiteof genesthatwerealsoregulatedbyinfection.Expressionprofilesoffivedifferentosmoticstressresistant(osr)mutantsunder isotonicconditionsreiteratedthewildtypetranscriptionalresponsetoosmoticstressandalsoshowedsubstantialsimilarity to infection-induced gene expression under isotonic conditions. Computational, transgenic, and functional approaches revealedthattwoGATAtranscriptionfactorspreviouslyimplicatedininfection-inducedtranscriptionalresponses,elt-2and elt-3, are also essential for coordinated tissue-specific activation of osmosensitive gene expression and promote survival under osmotically stressful conditions. Together, our data suggest infection and osmotic adaptation share previously unappreciated transcriptional similarities which might be controlled via regulation of tissue-specific GATA transcription factors. Citation:RohlfingA-K,MitevaY,HannenhalliS,LamitinaT(2010)GeneticandPhy