微生物燃料电池中脱色希瓦氏菌S12的产电特性研究.doc

微生物燃料电池中脱色希瓦氏菌S12的产电特性研究 李登兰1,2 许玫英1 孙国萍1* (1. 广东省微生物研究所广东省菌种保藏与应用重点实验室 广州 510070)(2. 广东工业大学环境科学与工程学院 广州 510090) 摘 要: 为了确定脱色希瓦氏菌S12的电化学活性, 采用循环伏安法(cyclic voltammograms, CV)对厌氧培养的菌株S12进行曲线扫描, 所得曲线表明S12具有一定的电化学活性, 可以用来进行产电实验。研究了不同电子供体和供体浓度对菌株S12产电的影响, 结果表明, 以浓度为10 mmol/L 的不同有机酸(甲酸钠、乳酸钠和丙酮酸钠)分别作为电子供体时, 乳酸钠产电量最大, 其最大功率密度Pmax为21.93 mW/m2, 增加乳酸钠的浓度, 菌株S12的产电量也相应增加, 当乳酸钠的浓度为20 mmol/L时, 所产生的最大功率密度达55.72 mW/m2。 关键词: 电化学活性, 产电, 电子供体, 功率密度 Electricity Generated by Shewanella decolorationnis S12 in Microbial Fuel Cell LI Deng-Lan1, 2 XU Mei-Ying1 SUN Guo-Ping1* (1. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou 510070) (2. Faculty of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510090) Abstract: In order to determine the electrochemical activity of Shewanella decolorationis S12, anaerobic grown cells were scanned by the cyclic voltammograms (CV). The results of CV showed that strain S12 displayed certain electrochemical activities and could be used as a catalyzer working in microbial fuel cell (MFC) to produce electricity. The effects of different electron donor, such as lactate, formate and pyruvate with different concentration on the production of electricity were analyzed by using strain S12 in a single chamber MFC with a PEM/electrode assembly sandwiched type. The results showed that lactate was the optimal electron donor and the concentration of the electron donor determined the electricity generation capacity and potential development in the MFC. When 10 mmol/L and 20 mmol/L of lactate were used, the maximum power density of 21.93 mW/m2 and 55.72 mW/m2 were obtained, respectively. Keywords: Electrochemical activity, Electricity generation, Electron donor, Power density 早在100年前就有研究者进行微生物产电的研究[1]。但微生物产电研究的再次受到重视, 却是在人们发现资源短缺、能源危机等对社会、经济的可持续发展存在潜在巨大威胁的最近几年[2,3]。 微生物燃料电池(microbial fuel cell, MFC)是一种利用微生物作为生物催化剂降解有机物将化学能转变为电能的装置[4,5