Mesenchymal Stem Cells Exhibit Firm Adhesion, Crawling, Spreading and Transmigration across Aortic Endothelial Cells Effects of Chemokines and Shear 英文参考文献.docVIP

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Mesenchymal Stem Cells Exhibit Firm Adhesion, Crawling, Spreading and Transmigration across Aortic Endothelial Cells Effects of Chemokines and Shear 英文参考文献.doc

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Mesenchymal Stem Cells Exhibit Firm Adhesion, Crawling, Spreading and Transmigration across Aortic Endothelial Cells Effects of Chemokines and Shear 英文参考文献

MesenchymalStemCellsExhibitFirmAdhesion, Crawling,SpreadingandTransmigrationacrossAortic EndothelialCells:EffectsofChemokinesandShear GiselleChamberlain1,HelenSmith1,G.EdRainger2,JimMiddleton1,3* 1LeopoldMullerArthritisResearchCentre,MedicalSchool,KeeleUniversity,RJAHOrthopaedicHospital,Oswestry,Shropshire,UnitedKingdom,2SchoolofClinicaland ExperimentalMedicine,CollegeofMedicineandDentistry,UniversityofBirmingham,Birmingham,UnitedKingdom,3FacultyofMedicineandDentistry,SchoolofOral andDentalSciences,UniversityofBristol,Bristol,UnitedKingdom Abstract Mesenchymal stem cells (MSCs) have anti-inflammatory and immunosuppressive properties and may be useful in the therapyofdiseasessuchasarteriosclerosis.MSCshavesomeabilitytotrafficintoinflamedtissues,howevertoexploitthis therapeutically their migratory mechanisms need to be elucidated. This study examines the interaction of murine MSCs (mMSCs)with,andtheirmigrationacross,murineaorticendothelialcells(MAECs),andtheeffectsofchemokinesandshear stress.TheinteractionofmMSCswithMAECswasexaminedunderphysiologicalflowconditions.mMSCsshowedlackof interactionwithMAECsundercontinuousflow.However,whentheflowwasstopped(for10min)andthenstarted,mMSCs adhered and crawled on the endothelial surface, extending fine microvillous processes (filopodia). They then spread extendingpseudopodiainmultipledirections.CXCL9significantlyenhancedthepercentageofmMSCsadhering,crawling andspreadingandshearforcesmarkedlystimulatedcrawlingandspreading.CXCL9,CXCL16,CCL20andCCL25significantly enhanced transendothelial migration across MAECs. The transmigrated mMSCs had down-regulated receptors CXCR3, CXCR6,CCR6andCCR9.ThisstudyfurtherstheknowledgeofMSCtransendothelialmigrationandtheeffectsofchemokines andshearstresswhichisofrelevancetoinflammatorydiseasessuchasarteriosclerosis. Citation:ChamberlainG,SmithH,RaingerGE,MiddletonJ(2011)MesenchymalStemCellsExhibitFirmAdhesion,Crawling,SpreadingandTransmigrationacross AorticEndothelialCells:EffectsofChemokinesandShea