Mycobacterium tuberculosis Complex Mycobacteria as Amoeba-Resistant Organisms 英文参考文献.docVIP

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Mycobacterium tuberculosis Complex Mycobacteria as Amoeba-Resistant Organisms 英文参考文献.doc

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Mycobacterium tuberculosis Complex Mycobacteria as Amoeba-Resistant Organisms 英文参考文献

MycobacteriumtuberculosisComplexMycobacteriaas Amoeba-ResistantOrganisms FelixMbaMedie1,2,IskandarBenSalah1,BernardHenrissat2,DidierRaoult1,MichelDrancourt1* 1Unite′ de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR 6236 CNRS - Universite′ de la Me′diterrane′e, IRD 189, IFR 48, Faculte′ de Me′decine, Marseille,France,2ArchitectureetFonctiondesMacromole′cules Biologiques,AFMB,UMR6098CNRS-Universite′ delaMe′diterrane′e,Marseille,France Abstract Background:Mostenvironmentalnon-tuberculousmycobacteriahavebeendemonstratedtoinvadeamoebaltrophozoites and cysts, but such relationships are largely unknown for members of the Mycobacterium tuberculosis complex. An environmentalsourcehasbeenproposedfortheanimalMycobacteriumbovisandthehumanMycobacteriumcanettii. Methodology/Principal Findings: Using optic and electron microscopy and co-culture methods, we observed that 8960.6%ofM.canettii,12.460.3%ofM.tuberculosis,11.762%ofM.bovisand11.260.5%ofMycobacteriumaviumcontrol organismswerephagocytizedbyAcanthamoebapolyphaga,aratiosignificantlyhigherforM.canettii(P=0.03),correlating withthesignificantlylargersizeofM.canettiorganisms(P=0.035).Thepercentageofintraamoebalmycobacteriasurviving intocytoplasmicvacuoleswas3262%forM.canettii,2661%forM.tuberculosis,2862%forM.bovisand3662%forM. avium(P=0.57).M.tuberculosis,M.bovisandM.aviummycobacteriawerefurtherentrappedwithinthedoublewallof,1% amoebalcysts,butnoM.canettiiorganismswereobservedinamoebalcysts.Thenumberofintracysticmycobacteriawas significantly(P=1026)higherforM.aviumthanfortheM.tuberculosiscomplex,andsub-culturingintracysticmycobacteria yielded significantly more (P=0.02) M. avium organisms (346104CFU/mL) than M. tuberculosis (426101CFU/mL) and M. bovis(35610 CFU/mL)inthepresenceofawashingfluidfreeofmycobacteria.Mycobacteriasurvivedinthecystsforupto 18 days and cysts protected M. tuberculosis organisms against mycobactericidal 5mg/mL streptomycin and 2.5% glutaraldehyde. 1 Conclusions/Significance: