Ordered Patterns of Cell Shape and Orientational Correlation during Spontaneous Cell Migration 英文参考文献.docVIP

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Ordered Patterns of Cell Shape and Orientational Correlation during Spontaneous Cell Migration 英文参考文献.doc

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Ordered Patterns of Cell Shape and Orientational Correlation during Spontaneous Cell Migration 英文参考文献

OrderedPatternsofCellShapeandOrientational CorrelationduringSpontaneousCellMigration YusukeT.Maeda.¤*,JunyaInose.,MikiY.Matsuo.,SuguruIwaya,MasakiSano DepartmentofPhysics,GraduateSchoolofScience,theUniversityofTokyo,Bunkyo-ku,Tokyo,Japan Abstract Background:Intheabsenceofstimuli,mostmotileeukaryoticcellsmovebyspontaneouslycoordinatingcelldeformation withcellmovementintheabsenceofstimuli.Yetlittleisknownabouthowcellschangetheirownshapeandhowcells coordinatethedeformationandmovement.Here,weinvestigatedthemechanismofspontaneouscellmigrationbyusing computationalanalyses. Methodology:WeobservedspontaneouslymigratingDictyosteliumcellsinbothavegetativestate(roundcellshapeand slowmotion)andstarvedone(elongatedcellshapeandfastmotion).Wethenextractedregularpatternsofmorphological dynamics and the pattern-dependent systematic coordination with filamentous actin (F-actin) and cell movement by statisticaldynamicanalyses. Conclusions/Significance:WefoundthatDictyosteliumcellsinbothvegetativeandstarvedstatescommonlyorganizetheir ownshapeintothreeorderedpatterns,elongation,rotation,andoscillation,intheabsenceofexternalstimuli.Further,cells inactivated for PI3-kinase (PI3K) and/or PTEN did not show ordered patterns due to the lack of spatial control in pseudopodial formation in both the vegetative and starved states. We also found that spontaneous polarization was achieved in starved cells by asymmetric localization of PTEN and F-actin. This breaking of the symmetry of protein localization maintained the leading edge and considerably enhanced the persistence of directed migration, and overall random exploration was ensured by switching among the different ordered patterns. Our findings suggest that DictyosteliumcellsspontaneouslycreatetheorderedpatternsofcellshapemediatedbyPI3K/PTEN/F-actinandcontrolthe directionofcellmovementbycoordinationwiththesepatternsevenintheabsenceofexternalstimuli. Citation: Maeda YT, Inose J, Matsuo MY, Iwaya S, Sano M (2008) Ordered Patterns of Cell Sha