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Production of engineered long-life and male sterile Pelargonium plants 英文参考文献
García-Sogoetal.BMCPlantBiology2012,12:156
/1471-2229/12/156
RESEARCH ARTICLE
OpenAccess
Productionofengineeredlong-lifeandmale
sterilePelargoniumplants
Bego?aGarcía-Sogo1,BenitoPineda1,EdelínRoque1,TeresaAntón1,AlejandroAtarés1,MariséBorja2,3,
JoséPíoBeltrán1,VicenteMoreno1andLuisAntonioCa?as1*
Abstract
Background:Pelargoniumisoneofthemostpopulargardenplantsintheworld.Moreover,ithasaconsiderable
economicimportanceintheornamentalplantmarket.Conventionalcross-breedingstrategieshavegenerateda
rangeofcultivarswithexcellenttraits.However,genetransferviaAgrobacteriumtumefacienscouldbeahelpfultool
tofurtherimprovePelargoniumbyenablingtheintroductionofnewgenes/traits.Wereportasimpleandreliable
protocolforthegenetictransformationofPelargoniumspp.andtheproductionofengineeredlong-lifeandmale
sterilePelargoniumzonaleplants,usingthepSAG12::iptandPsEND1::barnasechimaericgenesrespectively.
Results:ThepSAG12::ipttransgenicplantsshoweddelayedleafsenescence,increasedbranchingandreduced
internodallength,ascomparedtocontrolplants.LeavesandflowersofthepSAG12::iptplantswerereducedinsize
anddisplayedamoreintensecoloration.InthetransgeniclinescarryingthePsEND1::barnaseconstructnopollen
grainswereobservedinthemodifiedantherstructures,whichdevelopedinsteadofnormalanthers.Theloculesof
sterileantherscollapsed3–4dayspriortofloralanthesisand,inmostcases,theundevelopedanthertissues
underwentnecrosis.
Conclusion:ThechimaericconstructpSAG12::iptcanbeusefulinPelargoniumspp.todelaythesenescenceprocess
andtomodifyplantarchitecture.Inaddition,theuseofengineeredmalesterileplantswouldbeespeciallyuseful
toproduceenvironmentallyfriendlytransgenicplantscarryingnewtraitsbypreventinggeneflowbetweenthe
geneticallymodifiedornamentalsandrelatedplantspecies.Thesecharacteristicscouldbeofinterest,froma
commercialpointofview,bothforpelargoniumproducersandconsumers.
Keywords:Pelargoniumzonale,Pelargoniumpeltatum,pSAG12promoter,iptgene,Engineeredlong-livedplants,
Delayedsenescence,Engineeredmalesterility,PsEND1pro
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