Protective Effect of T. violacea Rhizome Extract Against Hypercholesterolemia-Induced Oxidative Stress in Wistar Rats 英文参考文献.docVIP

Molecules 2012, 17, 6033-6045; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Protective Effect of T. violacea Rhizome Extract Against Hypercholesterolemia-Induced Oxidative Stress in Wistar Rats Olorunnisola Sinbad Olorunnisola 1, Graeme Bradley 1 and Anthony Jide Afolayan 2,* 1 Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa; E-Mails: sinbadkd@ (O.S.O.); GBradley@ufh.ac.za (G.B.) 2 Phytomedicine Research Group, Department of Botany, University of Fort Hare, Alice 5700, South Africa * Author to whom correspondence should be addressed; E-Mail: aafolayan@ufh.ac.za; Tel./Fax: +27-86-628-2295. Received: 29 March 2012; in revised form: 27 April 2012 / Accepted: 28 April 2012 / Published: 21 May 2012 Abstract: The present study examines the effect of methanolic extract of T. violacea rhizomes on high cholesterol (2%) diet fed rats (HCD). At the end of 4 weeks, serum total protein, albumin, reduced glutathione (GSH), and markers of oxidative stress viz., catalase (CAT), superoxide dismutase (SOD), thiobarbituric acid reactive substances (TBARS— a marker of lipid peroxidation), glutathione-S-transferase (GST) and glutathione peroxidase (GPx) in the serum, aorta, liver and heart of HCD and normal rats were assessed and compared. A significant (p 0.05) elevation in TBARS, and a reduction (p 0.05) in serum total protein, albumin, GSH and antioxidant enzyme activities was observed in tissues of HCD fed rats compared with the normal group. Co-administration of crude extracts of T. violacea rhizomes protected the liver, heart, serum and aorta against HCD-induced lipid peroxidation in a dose dependant manner. The activities of the extract (500 mg/kg) compared favorably with gemfibrozil. The extracts also protected against HCD-induced reduction in serum total protein,