Proteomic Analysis of Excretory-Secretory Products of Heligmosomoides polygyrus Assessed with Next-Generation Sequencing Transcriptomic Information 英文参考文献.docVIP

下载本文档

9
0
约6.61万字
约 11页
2017-05-12 发布于上海
举报
版权申诉

Proteomic Analysis of Excretory-Secretory Products of Heligmosomoides polygyrus Assessed with Next-Generation Sequencing Transcriptomic Information 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Proteomic Analysis of Excretory-Secretory Products of Heligmosomoides polygyrus Assessed with Next-Generation Sequencing Transcriptomic Information 英文参考文献

ProteomicAnalysisofExcretory-SecretoryProductsof HeligmosomoidespolygyrusAssessedwithNext- GenerationSequencingTranscriptomicInformation YovanyMoreno1,Pierre-PaulGros2,MifongTam2,MarielaSegura2,RajeshValanparambil2,TimothyG. Geary1,MaryM.Stevenson2* 1InstituteofParasitologyandCentreforHostParasiteInteractions,McGillUniversity,Ste-AnnedeBellevue,Quebec,Canada,2CentrefortheStudyofHostResistance andCentreforHostParasiteInteractions,TheResearchInstituteofMcGillUniversityHealthCentreandDepartmentofMedicine,McGillUniversity,Montreal,Quebec, Canada Abstract The murine parasite Heligmosomoides polygyrus is a convenient experimental model to study immune responses and pathologyassociatedwithgastrointestinalnematodeinfections.Theexcretory-secretoryproducts(ESP)producedbythis parasitehavepotentimmunomodulatoryactivity,buttheprotein(s)responsiblehasnotbeendefined.Identificationofthe proteincompositionofESPderivedfromH.polygyrusandotherrelevantnematodespecieshasbeenhamperedbythelack of genomic sequence information required for proteomic analysis based on database searches. To overcome this, a transcriptome next generation sequencing (RNA-seq) de novo assembly containing 33,641 transcripts was generated, annotated,andusedtointerrogatemassspectrometry(MS)dataderivedfrom1D-SDSPAGEandLC-MS/MSanalysisofESP. Using the database generated from the 6 open reading frames deduced from the RNA-seq assembly and conventional identificationprograms,209proteinswereidentifiedinESPincludinghomologuesofvitellogenins,retinol-andfattyacid- bindingproteins,globins,andtheallergenV5/Tpx-1-relatedfamilyofproteins.Severalpotentialimmunomodulators,such as macrophage migration inhibitory factor, cysteine protease inhibitors, galectins, C-type lectins, peroxiredoxin, and glutathioneS-transferase,werealsoidentified.ComparativeanalysisofproteinannotationsbasedontheRNA-seqassembly and proteomics revealed processes and proteins that may contribute to the functional specialization of ESP, including proteins in