Pterins as Sensors of Response to the Application of Fe3+-Dextran in Piglets 英文参考文献.docVIP

Sensors 2010, 10, 890-900; doi:10.3390/s100100890 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Pterins as Sensors of Response to the Application of Fe3+-Dextran in Piglets Miriam Smutna 1,*, Martin Svoboda 2 and Klara Breinekova 3 1 Department of Biochemistry, Chemistry and Biophysics, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, Brno, 612 42, Czech Republic 2 Clinic of Pig Diseases, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, Brno, 612 42, Czech Republic; E-Mail: svobodama@vfu.cz 3 Synthon s. r. o., Brnenska 32, Blansko 678 17, Czech Republic; E-Mail: Klara.Breinekova@synthon.cz * Author to whom correspondence should be addressed; E-Mail: smutnam@vfu.cz; Tel.: +420-541-562-602; Fax: +420-541-562-790. Received: 16 November 2009; in revised form: 30 December 2009 / Accepted: 14 January 2010 / Published: 22 January 2010 Abstract: The aim of the presented study was to assess the effect of a single administration of Fe3+-dextran on immune cell counts and pterin biomolecule production as novel sensors of the piglets immune system activation, and to determine concentrations of cortisol, a traditional hormonal biosensor of the stress response. Pterins (neopterin and biopterin) in the piglets blood serum were analyzed by separation using reversed-phase HPLC. A single dose of Fe3+-dextran produced a special stress situation in the piglets organism which manifested itself by an increased production of neopterin (p 0.05) and biopterin (p 0.01) in the experimental piglets. Changes in cortisol concentrations and leukocyte counts were influenced by handling stress and were not specifically correlated to iron dextran application. Iron concentrations in the internal environment of the experimental piglets’ group were higher by an order of magnitude compare