Rapid Qualitative Urinary Tract Infection Pathogen Identification by SeptiFast？ Real-Time PCR 英文参考文献.docVIP

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Rapid Qualitative Urinary Tract Infection Pathogen Identification by SeptiFast？ Real-Time PCR 英文参考文献.doc

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Rapid Qualitative Urinary Tract Infection Pathogen Identification by SeptiFast？ Real-Time PCR 英文参考文献

RapidQualitativeUrinaryTractInfectionPathogen IdentificationbySeptiFastHReal-TimePCR LutzE.Lehmann1.,StefanHauser2.,ThomasMalinka3,SvenKlaschik4,StefanU.Weber4 ,Jens-Christian Schewe4,FrankStu¨ber1,MalteBook1* 1UniversityDepartmentofAnaesthesiologyandPainTherapy,Inselspital,BernUniversity,Bern,Switzerland,2ClinicandPoliclinicforUrology,UniversityHospitalBonn, Bonn, Germany, 3Kantonsspital Aarau AG, Department of Surgery, Aarau, Switzerland, 4University Department of Anaesthesiology and Intensive Care Medicine, UniversityHospitalBonn,Bonn,Germany Abstract Background: Urinary tract infections (UTI) are frequent in outpatients. Fast pathogen identification is mandatory for shorteningthetimeofdiscomfortandpreventingseriouscomplications.Urinecultureneedsupto48hoursuntilpathogen identification.Consequently,theinitialantibioticregimenisempirical. Aim:Toevaluatethefeasibilityofqualitativeurinepathogenidentificationbyacommerciallyavailablereal-timePCRblood pathogentest(SeptiFastH)andtocomparetheresultswithdipslideandmicrobiologicalculture. Designofstudy:Pilotstudywithprospectivelycollectedurinesamples. Setting:Universityhospital. Methods: 82 prospectively collected urine samples from 81 patients with suspected UTI were included. Dipslide urine culture was followed by microbiological pathogen identification in dipslide positive samples. In parallel, qualitative DNA basedpathogenidentification(SeptiFastH)wasperformedinallsamples. Results:61sampleswereSeptiFastHpositive,whereas67samplesweredipslideculturepositive.Theinter-methodological concordanceofpositiveandnegativefindingsinthegram+,gram-andfungisectorwas371/410(90%),477/492(97%)and 238/246(97%),respectively.SensitivityandspecificityoftheSeptiFastHtestforthedetectionofaninfectionwas0.82and 0.60,respectively.SeptiFastHpathogenidentificationswereavailableatleast43hourspriortocultureresults. Conclusion: The SeptiFastH platform identified bacterial DNA in urine specimens considerably faster compared to conventionalculture.For