Repetitive Element-Mediated Recombination as a Mechanism for New Gene Origination in Drosophila 英文参考文献.docVIP

RepetitiveElement-MediatedRecombination asaMechanismforNewGeneOrigination inDrosophila Shuang Yang1,2[,J.Roman Arguello3[,Xin Li1,2,Yun Ding1,2,QiZhou1,2,Ying Chen4,Yue Zhang1,Ruoping Zhao1, Fre′de′ricBrunet3¤,Lixin Peng1,Manyuan Long3,4*,Wen Wang1* 1 Chinese Academy of Sciences (CAS)—Max Planck Junior Research Group, Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese AcademyofSciences,Kunming,Yunnan,China2GraduateSchoolofChineseAcademySciences,Beijing,China,3CommitteeonEvolutionaryBiology,TheUniversityof Chicago,Chicago,Illinois,UnitedStatesofAmerica,4DepartmentofEcologyandEvolution,TheUniversityofChicago,Chicago,Illinois,UnitedStatesofAmerica Previousstudiesofrepetitiveelements(REs)haveimplicatedamechanisticroleingeneratingnewchimericalgenes. Such examples are consistent with the classic model for exon shuffling, which relies on non-homologous recombination. However, recent data for chromosomal aberrations in model organisms suggest that ectopic homology-dependent recombination may also be important. Lack of a dataset comprising experimentally verified young duplicates has hampered an effective examination of these models as well as an investigation of sequence features that mediate the rearrangements. Here we use ;7,000 cDNA probes (;112,000 primary images) to screen eightspecieswithin theDrosophila melanogastersubgroupandidentify17duplicates thatweregeneratedthrough ectopic recombination within the last 12 mys. Most of these are functional and have evolved divergent expression patterns and novel chimeric structures. Examination of their flanking sequences revealed an excess of repetitive sequences,withthemajoritybelongingtothetransposableelementDNAREP1family,associatedwiththenewgenes. OurdatasetstronglysuggestsanimportantroleforREsinthegenerationofchimericgeneswithinthesespecies. Citation:YangS,ArguelloJR,LiX,DingY,ZhouQ,etal.(2008)Repetitiveelement-mediatedrecombinationasamechanismfornewgeneoriginationinDroso