Restoration of Electric Footshock-Induced Immunosuppression in Mice by Gynostemma pentaphyllum Components 英文参考文献.docVIP

Molecules 2012, 17, 7695-7708; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Restoration of Electric Footshock-Induced Immunosuppression in Mice by Gynostemma pentaphyllum Components Sun-A Im, Hyun Sook Choi, Soon Ok Choi, Ki-Hyang Kim, Seungjeong Lee, Bang Yeon Hwang, Myung Koo Lee and Chong Kil Lee * College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea; E-Mails: sunaim@chungbuk.ac.kr (S.-A.I.); 6151494@ (H.S.C.); pa960001@cnuh.co.kr (S.O.C.); yes3343@ (K.-H.K.); chdang@ (S.L.); byhwang@chungbuk.ac.kr (B.Y.H.); myklee@chungbuk.ac.kr (M.K.L.) * Author to whom correspondence should be addressed; E-Mail: cklee@chungbuk.ac.kr; Tel.: +82-43-261-2826; Fax: +82-43-261-3571. Received: 17 February 2012; in revised form: 11 June 2012 / Accepted: 20 June 2012 / Published: 25 June 2012 Abstract: The immunomodulatory effects of the ethanol extract of Gynostemma pentaphyllum (GP-EX) were examined in electric footshock (EFS)-stressed mice. The mice were orally administered various doses of GP-EX for 7 days before exposure to EFS (duration: 3 min, interval: 10 s, intensity: 2 mA) once a day from day 8 for 14 days with continuous daily feeding of GP-EX. Oral administration of GP-EX to mice prevented EFS stress-induced immunosuppression as determined by the lymphoid organ (thymus and spleen) weight and cellularity. In addition, oral administration of GP-EX restored EFS-suppressed functional properties of mature lymphocytes in terms of concanavalin A-induced proliferation of splenocytes and lipopolysaccharide-induced cytokine production (TNF-α, IL-1β). Furthermore, we found that mice that were orally administered with GP-EX generated much more potent ovalbumin-specific cytotoxic T lymphocyte responses upon intravenous ovalbumin injection compared to the untreated controls. These results demonstrate that oral administr