Rheumatoid arthritis synovium contains plasmacytoid dendritic cells 英文参考文献.docVIP

Available online /content/7/2/R230 Research article Open Access Vol 7 No 2 Rheumatoid arthritis synovium contains plasmacytoid dendritic cells Lois L Cavanagh1, Amanda Boyce1, Louise Smith1, Jagadish Padmanabha1, Luis Filgueira2, Peter Pietschmann3 and Ranjeny Thomas1 1Centre for Immunology and Cancer Research, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia 2Institute of Anatomy, University Irchel-Zurich, Zurich, Switzerland 3Department of General and Experimental Pathology, University of Vienna, Vienna, Austria Corresponding author: Ranjeny Thomas, rthomas@.au Received: 11 Jun 2004 Revisions requested: 29 Jul 2004 Revisions received: 13 Oct 2004 Accepted: 26 Oct 2004 Published: 11 Jan 2005 Arthritis Res Ther 2005, 7:R230-R240 (DOI 10.1186/ar1467) ? 2004 Cavanagh et al.; licensee BioMed Central Ltd. /content/7/2/R230 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/ 2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is cited. Abstract We have previously described enrichment of antigen-presenting HLA-DR+ nuclear RelB+ dendritic cells (DCs) in rheumatoid arthritis (RA) synovium. CD123+HLA-DR+ plasmacytoid DCs (pDCs) and their precursors have been identified in human peripheral blood (PB), lymphoid tissue, and some inflamed tissues. We hypothesized recruitment of pDCs into the inflamed RA synovial environment and their contribution as antigen- presenting cells (APCs) and inflammatory cells in RA. CD11c+ nuclear RelB+CD123- DCs, but not normal synovial tissue sublining. Apart from higher expression of HLA-DR, the numbers and phenotypes of SF pDCs were similar to those of normal PB pDCs. While the APC function of PB pDCs was less efficient than that of PB myeloid DCs, RA SF pDCs efficiently activated resting allogeneic PB T cells, and high levels of IFN-γ, IL-10, a