RNA-Interference Knockdown of Drosophila Pigment Dispersing Factor in Neuronal Subsets The Anatomical Basis of a Neuropeptides Circadian Functions 英文参考文献.docVIP

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RNA-Interference Knockdown of Drosophila Pigment Dispersing Factor in Neuronal Subsets The Anatomical Basis of a Neuropeptides Circadian Functions 英文参考文献.doc

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RNA-Interference Knockdown of Drosophila Pigment Dispersing Factor in Neuronal Subsets The Anatomical Basis of a Neuropeptides Circadian Functions 英文参考文献

RNA-InterferenceKnockdownofDrosophilaPigment DispersingFactorinNeuronalSubsets:TheAnatomical BasisofaNeuropeptide’sCircadianFunctions OrieT.Shafer¤*,PaulH.Taghert DepartmentofAnatomyandNeurobiology,WashingtonUniversityMedicalSchool,St.Louis,Missouri,UnitedStatesofAmerica Abstract Background:Inanimals,neuropeptidesignalingisanimportantcomponentofcircadiantimekeeping.Theneuropeptide pigmentdispersingfactor(PDF)isrequiredforseveralaspectsofcircadianactivityrhythmsinDrosophila. Methodology/Principal Findings: Here we investigate the anatomical basis for PDF’s various circadian functions by targetedPDFRNA-interferenceinspecificclassesofDrosophilaneuron.WedemonstratethatPDFisrequiredintheventro- lateral neurons (vLNs) of the central brain and not in the abdominal ganglion for normal activity rhythms. Differential knockdown of PDF in the large or small vLNs indicates that PDF from the small vLNs is likely responsible for the maintenanceoffree-runningactivityrhythmsandthatPDFisnotrequiredinthelargevLNsfornormalbehavior.PDF’srole in setting the period of free-running activity rhythms and the proper timing of evening activity under light:dark cycles emanatesfrombothsubtypesofvLN,sincePDFineitherclassofvLNwassufficientfortheseaspectsofbehavior. Conclusions/Significance:TheseresultsrevealtheneuroanatomicalbasisPDF’svariouscircadianfunctionsandrefineour understandingoftheclockneuroncircuitryofDrosophila. Citation:ShaferOT,TaghertPH(2009)RNA-InterferenceKnockdownofDrosophilaPigmentDispersingFactorinNeuronalSubsets:TheAnatomicalBasisofa Neuropeptide’sCircadianFunctions.PLoSONE4(12):e8298.doi:10.1371/journal.pone.0008298 Editor:MichaelN.Nitabach,YaleSchoolofMedicine,UnitedStatesofAmerica ReceivedOctober22,2009;AcceptedNovember17,2009;PublishedDecember14,2009 Copyright: ? 2009 Shafer, Taghert. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheorigi