Routine Habitat Change A Source of Unrecognized Transient Alteration of Intestinal Microbiota in Laboratory Mice 英文参考文献.docVIP

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Routine Habitat Change A Source of Unrecognized Transient Alteration of Intestinal Microbiota in Laboratory Mice 英文参考文献.doc

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Routine Habitat Change A Source of Unrecognized Transient Alteration of Intestinal Microbiota in Laboratory Mice 英文参考文献

RoutineHabitatChange:ASourceofUnrecognized TransientAlterationofIntestinalMicrobiotain LaboratoryMice BettyW.Ma1,2,NicholasA.Bokulich3,4,PatriciaA.Castillo2,AnchasaKananurak2,MarkA.Underwood5, DavidA.Mills3,4,CharlesL.Bevins2* 1CenterforLaboratoryAnimalScience,SchoolofVeterinaryMedicine-ResidencyPrograminLaboratoryAnimalMedicine,UniversityofCaliforniaDavis,Davis,California, UnitedStatesofAmerica,2DepartmentofMicrobiologyandImmunology,SchoolofMedicine,UniversityofCaliforniaDavis,Davis,California,UnitedStatesofAmerica, 3Department of Food Science and Technology, University of California Davis, Davis, California, United States of America, 4Department of Viticulture and Enology, University of California Davis, Davis, California, United States of America, 5Department of Pediatrics, School of Medicine, University of California Davis, Sacramento, California,UnitedStatesofAmerica Abstract Themammalianintestineharborsavast,complexanddynamicmicrobialpopulation,whichhasprofoundeffectsonhost nutrition, intestinal function and immune response, as well as influence on physiology outside of the alimentary tract. Imbalanceinthecompositionofthedensecolonizingbacterialpopulationcanincreasesusceptibilitytovariousacuteand chronicdiseases.Valuableinsightsontheassociationofthemicrobiotawithdiseasecriticallydependoninvestigationof mousemodels.Likeinhumans,themicrobialcommunityinthemouseintestineisrelativelystableandresilient,yetcanbe influenced by environmental factors. An often-overlooked variable in research is basic animal husbandry, which can potentially alter mouse physiology and experimental outcomes. This study examined the effects of common husbandry practices,includingfoodandbeddingalterations,aswellasfacilityandcagechanges,onthegutmicrobiotaoverashort timecourseoffivedaysusingthreeculture-independenttechniques,quantitativePCR,terminalrestrictionfragmentlength polymorphism (TRFLP) and next generation sequencing (NGS). This study detected a substantial transient alteration in mi