Role of the trigeminal mesencephalic nucleus in rat whisker pad proprioception 英文参考文献.docVIP
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Role of the trigeminal mesencephalic nucleus in rat whisker pad proprioception 英文参考文献
Mamelietal.BehavioralandBrainFunctions2010,6:69
/content/6/1/69
RESEARCH
OpenAccess
Roleofthetrigeminalmesencephalicnucleusin
ratwhiskerpadproprioception
OmbrettaMameli1*,StefaniaStanzani2,GabrieleMulliri1,RosaliaPellitteri3,MarcelloACaria1,
AntonellaRusso2,PierluigiDeRiu1
Abstract
Background:Trigeminalproprioceptionrelatedtorodentmacrovibrissaemovementsisbelievedtoinvolveskin
receptorsonthewhiskerpadbecausepadmusclesoperatewithoutmusclespindles.Thisstudywasaimedto
investigateinratswhetherthetrigeminalmesencephalicnucleus(TMnu),whichprovidesproprioceptivefeedback
forchewingmuscles,maybealsoinvolvedinwhiskerpadproprioception.
Methods:Tworetrogradetracers,DilandTrueBlueChloride,wereinjectedintothemystacialpadandthe
massetermuscleonthesamesideofdeeplyanesthetizedratstolabeltherespectiveprojectingsensoryneurons.
Thisdouble-labelingtechniquewasusedtoassesstheco-innervationofbothstructuresbythetrigeminal
mesencephalicnucleus(TMnu).
Inaseparategroupofanesthetizedanimals,thespontaneouselectricalactivitiesofTMnuneuronswereanalyzed
byextracellularrecordingsduringspontaneousmovementsofthemacrovibrissae.Mesencephalicneurons(TMne)
werepreviouslyidentifiedbytheirresponsestomassetermusclestretching.ChangesinTMnespontaneouselectri-
calactivities,analyzedunderbaselineconditionsandduringwhiskingmovements,werestatisticallyevaluated
usingStudent’st-testforpairedobservations.
Results:Neuroanatomicalexperimentsrevealeddifferentsubpopulationsoftrigeminalmesencephalicneurons:i)
thoseinnervatingtheneuromuscularspindlesofthemassetermuscle,ii)thoseinnervatingthemystacialpad,and
iii)thoseinnervatingbothstructures.Extracellularrecordingsmadeduringspontaneousmovementsofthe
macrovibrisaeshowedthatwhiskingneuronssimilartothoseobservedinthetrigeminalganglionwerelocatedin
theTMnu.Theseneuronshaddifferentpatternsofactivation,whichweredependentonthetypeofspontaneous
macrovibrissaemovement.Inparticular,theirspikingactivitytonicallyincreasedduringfan-likemovementsofthe
vibrissaeandshowedphasicbursting
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