Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells 英文参考文献.docVIP

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Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells 英文参考文献.doc

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Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells 英文参考文献

BMC Plant Biology BioMedCentral Research article Open Access Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells Mari Aidemark, Carl-Johan Andersson, Allan G Rasmusson and Susanne Widell* Address: Department of Cell and Organism Biology, Lund University, S?lvegatan 35, SE-223 62 Lund, Sweden Email: Mari Aidemark - mari.Aidemark@cob.lu.se; Carl-Johan Andersson - cajo.andersson@; Allan G Rasmusson - Allan.Rasmusson@cob.lu.se; Susanne Widell* - Susanne.Widell@cob.lu.se * Corresponding author Published: 12 March 2009 Received: 3 October 2008 Accepted: 12 March 2009 BMC Plant Biology 2009, 9:27 doi:10.1186/1471-2229-9-27 This article is available from: /1471-2229/9/27 ? 2009 Aidemark et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The cell wall component callose is mainly synthesized at certain developmental stages and after wounding or pathogen attack. Callose synthases are membrane-bound enzymes that have been relatively well characterized in vitro using isolated membrane fractions or purified enzyme. However, little is known about their functional properties in situ, under conditions when the cell wall is intact. To allow in situ investigations of the regulation of callose synthesis, cell suspensions of Arabidopsis thaliana (Col-0), and tobacco (BY-2), were permeabilized with the channel-forming peptide alamethicin. Results: Nucleic acid-binding dyes and marker enzymes demonstrated alamethicin permeabilization of plasma membrane, mitochondria and plastids, also allowing callose synthase measurements. In the presence of alamethicin, Ca2+ addition was required for callose synthase a