Lowe Syndrome Protein OCRL1 Supports Maturation of Polarized Epithelial Cells 英文参考文献.docVIP

下载本文档

8
0
约6.38万字
约 13页
2017-05-13 发布于上海
举报
版权申诉

Lowe Syndrome Protein OCRL1 Supports Maturation of Polarized Epithelial Cells 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Lowe Syndrome Protein OCRL1 Supports Maturation of Polarized Epithelial Cells 英文参考文献

LoweSyndromeProteinOCRL1SupportsMaturationof PolarizedEpithelialCells AdamG.Grieve1.¤,RachelD.Daniels1.,ElenaSanchez-Heras1,MatthewJ.Hayes1,StephenE.Moss1, KarlMatter1,MartinLowe2,TimothyP.Levine1* 1Department of Cell Biology, UCL Institute of Ophthalmology, London, United Kingdom, 2Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom Abstract Mutations in the inositol polyphosphate 5-phosphatase OCRL1 cause Lowe Syndrome, leading to cataracts, mental retardationandrenalfailure.WenotedthatcelltypesaffectedinLoweSyndromearehighlypolarized,andthereforewe studiedOCRL1inepithelialcellsastheymaturefromisolatedindividualcellsintopolarizedsheetsandcystswithextensive communicationbetweenneighbouringcells.WeshowthataproportionofOCRL1targetsintercellularjunctionsattheearly stagesoftheirformation,co-localizingbothwithadherensjunctionalcomponentsandwithtightjunctionalcomponents. Correlating with this distribution, OCRL1 forms complexes with junctional components a-catenin and zonula occludens (ZO)-1/2/3.DepletionofOCRL1inepithelialcellsgrowingasasheetinhibitsmaturation;cellsremainflat,failtopolarize apicalmarkersandalsoshowreducedproliferation.Theeffectonshapeisrevertedbyre-expressedOCRL1andrequiresthe 59-phosphatase domain, indicating that down-regulation of 5-phosphorylated inositides is necessary for epithelial development.TheeffectofOCRL1inepithelialmaturationisseenmorestronglyin3-dimensionalcultures,whereepithelial cellslackingOCRL1notonlyfailtoformacentrallumen,butalsodonothavethecorrectintracellulardistributionofZO-1, suggestingthatOCRL1functionsearlyinthematurationofintercellularjunctionswhencellsgrowascysts.AroleofOCRL1 injunctionsofpolarizedcellsmayexplainthepatternoforgansaffectedinLoweSyndrome. Citation:GrieveAG,DanielsRD,Sanchez-HerasE,HayesMJ,MossSE,etal.(2011)LoweSyndromeProteinOCRL1SupportsMaturationofPolarizedEpithelial Cells.PLoSONE6(8):e24044.doi:10.1371/journal.pone.0024044 Editor:QuanshengDu,GeorgiaHealthSciencesUniversity,Unite