ruvA Mutants That Resolve Holliday Junctions but Do Not Reverse Replication Forks 英文参考文献.docVIP

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ruvA Mutants That Resolve Holliday Junctions but Do Not Reverse Replication Forks 英文参考文献.doc

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ruvA Mutants That Resolve Holliday Junctions but Do Not Reverse Replication Forks 英文参考文献

ruvAMutantsThatResolveHollidayJunctionsbutDo NotReverseReplicationForks ZeynepBaharoglu1,2,3,AlisonSylviaBradley4,MarieLeMasson1,2,3,IrinaTsaneva4,Be′ne′dicte Michel1,2,3 * 1CNRS,CentredeGe′ne′tiqueMole′culaire,UPR2167,Gif-sur-Yvette,France,2Universite′ Paris-Sud,Orsay,France,3Universite′ PierreetMarieCurie-Paris6,Paris,France, 4UCLDepartmentofBiochemistryandMolecularBiology,UniversityCollegeLondon,London,UnitedKingdom Abstract RuvABandRuvABCcomplexescatalyzebranchmigrationandresolutionofHollidayjunctions(HJs)respectively.Inaddition totheiractioninthelaststepsofhomologousrecombination,theyprocessHJsmadebyreplicationforkreversal,areaction which occurs at inactivated replication forks by the annealing of blocked leading and lagging strand ends. RuvAB was recentlyproposedtobindreplicationforksanddirectlycatalyzetheirconversionintoHJs.Wereportheretheisolationand characterization of two separation-of-function ruvA mutants that resolve HJs, based on their capacity to promote conjugational recombination and recombinational repair of UV and mitomycin C lesions, but have lost the capacity to reverseforks.InvivoandinvitroevidenceindicatethattheruvAmutationsaffectDNAbindingandthestimulationofRuvB helicase activity. This work shows that RuvA’s actions at forks and at HJs can be genetically separated, and that RuvA mutantscompromisedforforkreversalremainfullycapableofhomologousrecombination. Citation: Baharoglu Z, Bradley AS, Le Masson M, Tsaneva I, Michel B (2008) ruvA Mutants That Resolve Holliday Junctions but Do Not Reverse Replication Forks.PLoSGenet4(3):e1000012.doi:10.1371/journal.pgen.1000012 Editor:WilliamF.Burkholder,StanfordUniversity,UnitedStatesofAmerica ReceivedNovember29,2007;AcceptedJanuary28,2008;PublishedMarch7,2008 Copyright: ? 2008 Baharoglu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsou