Scapinin, the Protein Phosphatase 1 Binding Protein, Enhances Cell Spreading and Motility by Interacting with the Actin Cytoskeleton 英文参考文献.docVIP

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Scapinin, the Protein Phosphatase 1 Binding Protein, Enhances Cell Spreading and Motility by Interacting with the Actin Cytoskeleton 英文参考文献.doc

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Scapinin, the Protein Phosphatase 1 Binding Protein, Enhances Cell Spreading and Motility by Interacting with the Actin Cytoskeleton 英文参考文献

Scapinin,theProteinPhosphatase1BindingProtein, EnhancesCellSpreadingandMotilitybyInteractingwith theActinCytoskeleton JunjiSagara1*,ToshiakiArata3,ShunichiroTaniguchi2 1Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University, Matsumoto, Nagano, Japan, 2Department of Molecular Oncology, Graduate School of Medicine, Shinshu University, Matsumoto, Nagano, Japan, 3Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka,Osaka,Japan Abstract Scapinin,alsonamedphactr3,isanactinandproteinphosphatase1(PP1)bindingprotein,whichisexpressedintheadult brainandsometumorcells.Atpresent,therole(s)ofscapinininthebrainandtumorsarepoorlyunderstood.Weshowthat theRPEL-repeatdomainofscapinin,whichisresponsibleforitsdirectinteractionwithactin,inhibitsactinpolymerizationin vitro.Next,weestablishedaHelacellline,wherescapininexpressionwasinducedbytetracycline.Inthesecells,expression ofscapininstimulatedcellspreadingandmotility.Scapininwascolocalizedwithactinattheedgeofspreadingcells.To explore the roles of the RPEL-repeat and PP1-binding domains, we expressed wild-type and mutant scapinins as fusion proteins with green fluorescence protein (GFP) in Cos7 cells. Expression of GFP-scapinin (wild type) also stimulated cell spreading,butmutationintheRPEL-repeatdomainabolishedboththeactinbindingandthecellspreadingactivity.PP1- binding deficient mutants strongly induced cell retraction. Long and branched cytoplasmic processes were developed during the cell retraction. These results suggest that scapinin enhances cell spreading and motility through direct interactionwithactinandthatPP1playsaregulatoryroleinscapinin-inducedmorphologicalchanges. Citation:SagaraJ,ArataT,TaniguchiS(2009)Scapinin,theProteinPhosphatase1BindingProtein,EnhancesCellSpreadingandMotilitybyInteractingwiththe ActinCytoskeleton.PLoSONE4(1):e4247.doi:10.1371/journal.pone.0004247 Editor:RobinCharlesMay,UniversityofBirmingham,UnitedKingdom ReceivedSeptember18,200