Saponin accumulation in the seedling root of Panax notoginseng 英文参考文献.docVIP

Saponin accumulation in the seedling root of Panax notoginseng 英文参考文献.doc

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Saponin accumulation in the seedling root of Panax notoginseng 英文参考文献

Wangetal.ChineseMedicine2011,6:5 /content/6/1/5 RESEARCH OpenAccess Saponinaccumulationintheseedlingrootof Panaxnotoginseng DongWang,HongtaoZhu,KekeChen,MinXu,YingjunZhang*,ChongrenYang Abstract Background:PanaxnotoginsengisanimportantChinesemedicinalplant.Dammarene-typetriterpenoidsaponins aremainpharmacologicallyeffectivecompoundsinP.notoginseng.Thisstudyaimstoinvestigatetheformation andaccumulationofsaponinsinP.notoginsengrootsduringgerminationandjuvenilestage. Methods:P.notoginsengseedswerecollectedandstoredinwetsand.Aftergermination,theseedlingswere transplantedintoasoilnurserybedandcultivatedforoneyear.Duringthisperiod,sampleswerecollectedevery monthandtheconcentrationsofginsengnosidesRg1,Re,Rb1,RdandnotoginsengnosideR1weredeterminedby HPLC. Results:TherewaslittlesaponinintheP.notoginsengseed.Thechemicalcompositionofseedwasdifferentfrom thatofroot.Aftergermination,Rb1,Rg1,Re,RdandR1appearedsuccessivelyintheseedlingroot.Andinthefive- month-oldroot,allthesefivemainsaponinscameintoexistence.TheaccumulationofsaponinsinP.notoginseng rootwasaffectedbyseasons. Conclusion:Theaccumulationofsaponinsshowedatime-dependentincreaseaftergerminationofP.notoginseng. Background development.Thepresentstudyaimstoinvestigate the Panaxnotoginseng(Burk.)F.H.Chen.(Sanqi),aspecies formation andaccumulation ofsaponin constituents in belongingtotheAraliaceaefamily,isanimportantmed- P. notoginseng during germination and juvenile stages, icinalplantforitshaemostaticandrestorativeproperties particularly thedevelopmental changesofRg1,Re,Rb1, [1]. Much chemical and pharmacological research on RdandR1intheseedandseedlingroot. P. notoginseng has been carried out, indicating that dammarene-typetriterpenoidsaponinsarenotonlythe Methods main chemical components butalso themainpharma- Solvents and chemicals cologically effective components. They exert various Methanol(MeOH)waspurchasedfromTianjingchemi- effects on the cardiocerebral vascular system, central cal Ltd (China

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