Sarcomere length-dependence of activity-dependent twitch potentiation in mouse skeletal muscle 英文参考文献.docVIP

BMC Physiology BioMedCentral Research article Open Access Sarcomere length-dependence of activity-dependent twitch potentiation in mouse skeletal muscle Dilson E Rassier and Brian R MacIntosh* Address: Human Performance Laboratory, Faculty of Kinesiology, University of Calgary, 2500 University Drive, T2N 1N4, Calgary (AB), Canada Email: Dilson E Rassier - rassier@kin.ucalgary.ca; Brian R MacIntosh* - brian@kin.ucalgary.ca * Corresponding author Published: 10 December 2002 Received: 18 April 2002 Accepted: 10 December 2002 BMC Physiology 2002, 2:19 This article is available from: /1472-6793/2/19 ? 2002 Rassier and MacIntosh; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permit- ted in all media for any purpose, provided this notice is preserved along with the articles original URL. staircaseposttetanic potentiationmyosin light chain phosphorylation Abstract Background: It has been reported that potentiation of a skeletal muscle twitch response is proportional to muscle length with a negative slope during staircase, and a positive slope during posttetanic potentiation. This study was done to directly compare staircase and posttetanic responses with measurement of sarcomere length to compare their length-dependence. Methods: Mouse extensor digitorum longus (EDL) muscles were dissected to small bundles of fibers, which permit measurement of sarcomere length (SL), by laser diffraction. In vitro fixed-end contractions of EDL fiber bundles were elicited at 22°C and 35°C at sarcomere lengths ranging from 2.35 μm to 3.85 μm. Twitch contractions were assessed before and after 1.5 s of 75 Hz stimulation at 22°C or during 10 s of 10 Hz stimulation at 22°C or 35°C. Results: Staircase potentiation was greater at 35°C than 22°C, and the relative magnitude of the twitch contraction (Pt*/Pt) was proportional to sarcomere length with a n