Screening of Anti-Obesity Agent from Herbal Mixtures 英文参考文献.docVIP

Molecules 2012, 17, 3630-3638; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Communication Screening of Anti-Obesity Agent from Herbal Mixtures Changhyun Roh *, Uhee Jung and Sung-Kee Jo Division of Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 1266, Sinjeong-dong, Jeongeup, Jeonbuk 580-185, Korea; E-Mails: uhjung@kaeri.re.kr (U.J.); skjo@kaeri.re.kr (S.-K.J.) * Author to whom correspondence should be addressed; E-Mail: chroh@kaeri.re.kr; Tel.: +82-63-570-3133; Fax: +82-63-570-3139. Received: 2 March 2012; in revised form: 19 March 2012 / Accepted: 21 March 2012 / Published: 23 March 2012 Abstract: Globally, one in three of the World’s adults are overweight and one in 10 is obese. By 2015, World Health Organization (WHO) estimates the number of chubby adults will balloon to 2.3 billion—Equal to the combined populations of China, Europe and the United States. The discovery of bioactive compounds from herbs is one possible way to control obesity and to prevent or reduce the risks of developing various obesity-related diseases. In this study, we screened anti-obesity agents such as methyl gallate from the herbal composition known as HemoHIM that actively inhibits lipid formation as evidenced by Oil Red O staining and triglyceride (TG) contents in 3T3-L1 adipocytes, suggesting their use as an anti-obesity agent. Furthermore, the amount of glycerol released from cells into the medium had increased by treatment of methyl gallate in a concentration-dependent manner. The present study suggests that a promising anti-obesity agent like methyl gallate might be of therapeutic interest for the treatment of obesity. Keywords: anti-obesity; screening; methyl gallate; HemoHIM; lipid inhibition 1. Introduction Obesity is a leading preventable cause of death worldwide, with increasing prevalence in adults and children, and aut