Screening of Aptamers on Microfluidic Systems for Clinical Applications 英文参考文献.docVIP

Sensors 2012, 12, 9514-9529; doi:10.3390/s120709514 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review Screening of Aptamers on Microfluidic Systems for Clinical Applications Chen-Hsun Weng 1, Chao-Jyun Huang 2 and Gwo-Bin Lee 3,* 1 Department of Microbiology and Immunology, National Cheng Kung University, Tainan 70101, Taiwan; E-Mail: 2 Department of Mechanical Engineering, National Taiwan University, Taipei 10617, Taiwan; E-Mail: c.j.huang0706@ 3 Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan * Author to whom correspondence should be addressed; E-Mail: gwobin@.tw; Tel: +886-3-571-5131 (ext. 33765); Fax: +886-3-572-2840. Received: 30 May 2012; in revised form: 2 July 2012 / Accepted: 6 July 2012 / Published: 11 July 2012 Abstract: The use of microfluidic systems for screening of aptamers and their biomedical applications are reviewed in this paper. Aptamers with different nucleic acid sequences have been extensively studied and the results demonstrated a strong binding affinity to target molecules such that they can be used as promising candidate biomarkers for diagnosis and therapeutics. Recently, the aptamer screening protocol has been conducted with microfluidic-based devices. Furthermore, aptamer affinity screening by a microfluidic-based method has demonstrated remarkable advantages over competing traditional methods. In this paper, we first reviewed microfluidic systems which demonstrated efficient and rapid screening of a specific aptamer. Then, the clinical applications of screened aptamers, also performed by microfluidic systems, are further reviewed. These automated microfluidic systems can provide advantages over their conventional counterparts including more compactness, faster analysis, less sample/reagent consumption and automation. An aptamer-based compact microfluidic system for diagnosis may even lea