Sequence Motifs in MADS Transcription Factors Responsible for Specificity and Diversification of Protein-Protein Interaction 英文参考文献.docVIP

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Sequence Motifs in MADS Transcription Factors Responsible for Specificity and Diversification of Protein-Protein Interaction 英文参考文献.doc

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SequenceMotifsinMADSTranscriptionFactors ResponsibleforSpecificityandDiversificationofProtein- ProteinInteraction AaltD.J.vanDijk1,GiuseppaMorabito2¤,MartijnFiers1,RoelandC.H.J.vanHam1,GercoC. Angenent1,2,RichardG.H.Immink1,2 * 1PlantResearchInternational,Bioscience,Wageningen,TheNetherlands,2CentreforBioSystemsGenomics(CBSG),Wageningen,TheNetherlands Abstract Protein sequencesencompass tertiarystructures andcontain informationaboutspecific molecularinteractions, whichin turndeterminebiologicalfunctionsofproteins.Knowledgeabouthowproteinsequencesdefineinteractionspecificityis largelymissing,inparticularforparalogousproteinfamilieswithhighsequencesimilarity,suchastheplantMADSdomain transcription factor family. In comparison to the situation in mammalian species, this important family of transcription regulators has expanded enormously in plant species and contains over 100 members in the model plant species Arabidopsisthaliana.Here,weprovideinsightintothemechanismsthatdetermineprotein-proteininteractionspecificityfor theArabidopsisMADSdomaintranscriptionfactorfamily,usinganintegratedcomputationalandexperimentalapproach. Plant MADS proteins have highly similar amino acid sequences, but their dimerization patterns vary substantially. Our computationalanalysisuncoveredsmallsequenceregionsthatexplainobserveddifferencesindimerizationpatternswith reasonable accuracy. Furthermore, we show the usefulness of the method for prediction of MADS domain transcription factor interaction networks in other plant species. Introduction of mutations in the predicted interaction motifs demonstratedthatsingleaminoacidmutationscanhavealargeeffectandleadtolossorgainofspecificinteractions.In addition, various performed bioinformatics analyses shed light on the way evolution has shaped MADS domain transcriptionfactorinteractionspecificity.Identifiedprotein-proteininteractionmotifsappearedtobestronglyconserved amongorthologs,indicatingtheirevolutionaryimportance.Wealsoprovideevidencethatm