Serum MicroRNAs as Biomarkers for Hepatocellular Carcinoma in Chinese Patients with Chronic Hepatitis B Virus Infection 英文参考文献.docVIP

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Serum MicroRNAs as Biomarkers for Hepatocellular Carcinoma in Chinese Patients with Chronic Hepatitis B Virus Infection 英文参考文献.doc

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Serum MicroRNAs as Biomarkers for Hepatocellular Carcinoma in Chinese Patients with Chronic Hepatitis B Virus Infection 英文参考文献

SerumMicroRNAsasBiomarkersforHepatocellular CarcinomainChinesePatientswithChronicHepatitisB VirusInfection PengQi1.,Shu-qunCheng2.,HaoWang3,NanLi2,Yue-fengChen1,Chun-fangGao1* 1DepartmentofLaboratory Medicine,SecondMilitaryMedicalUniversity,EasternHepatobiliaryHospital,Shanghai,China,2DepartmentofOncologyComprehensive Treatment,SecondMilitaryMedicalUniversity,EasternHepatobiliaryHospital,Shanghai,China,3DepartmentofLaboratoryMedicine,SecondMilitaryMedicalUniversity, ChangzhengHospital,Shanghai,China Abstract Background:MicroRNAs(miRNAs)havebeenshowntoanticipategreatcancerdiagnosticpotential.Recently,circulating miRNAshavebeenreportedaspromisingbiomarkersforvariouspathologicconditions.Theobjectiveofthisstudywasto investigatethepotentialofserummiRNAsasnovelbiomarkersforhepatocellularcarcinoma(HCC). Methodology/PrincipalFindings:Thisstudywasdividedintofourphases:(I)TencandidateserummiRNAsweredetected byusingreal-timeRT-PCR,corresponding10HCCpatientswithchronichepatitisBvirus(HBV)infectionand10age-and sex-matched healthy subjects. (II) Marker validation by real-time RT-PCR on HBV patients with (n=48) or without HCC (n=48),andhealthysubjects(n=24).(III)Markerdetectionbyreal-timeRT-PCRinserafromanother14HCCpatientsbefore and1monthaftersurgicalresection.(IV)WeexaminedthecorrelationbetweentheexpressionsofcandidateserummiRNAs withclinicalparametersofHCCpatients.AlthoughmiR-222,miR-223ormiR-21weresignificantlyup-ordown-regulated betweenHCCpatientsandhealthycontrols,nosignificantdifferencewasobservedinthelevelsofthesemiRNAsbetween HBV patients without and with HCC. MiR-122 in serum was significantly higher in HCC patients than healthy controls (p,0.001).Moreimportantly,itwasfoundthatthelevelsofmiR-122weresignificantlyreducedinthepost-operativeserum sampleswhencomparedtothepre-operativesamples.AlthoughserummiR-122wasalsoelevatedinHBVpatientswith HCCcomparingwiththosewithoutHCC,thedifferencewasattheborderline(p=0.043). Conclusions/Significance: Our results suggest that serum