Sex Pheromone Receptor Specificity in the European Corn Borer Moth, Ostrinia nubilalis 英文参考文献.docVIP
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Sex Pheromone Receptor Specificity in the European Corn Borer Moth, Ostrinia nubilalis 英文参考文献
SexPheromoneReceptorSpecificityintheEuropean
CornBorerMoth,Ostrinianubilalis
KevinW.Wanner1*,AndrewS.Nichols2,JeanE.Allen1,PeggyL.Bunger1,StephenF.Garczynski3,
CharlesE.Linn,Jr.4,HughM.Robertson5,CharlesW.Luetje2
1DepartmentofPlantSciencesandPlantPathology,MontanaStateUniversity,Bozeman,Montana,UnitedStatesofAmerica, 2DepartmentofMolecularandCellular
Pharmacology,MillerSchoolofMedicine,UniversityofMiami,Miami,Florida,UnitedStatesofAmerica,3YakimaAgriculturalResearchLaboratory,AgriculturalResearch
Service, United States Department of Agriculture, Wapato, Washington, United States of America, 4Department of Entomology, Barton Laboratory, New York State
AgriculturalExperimentStation,CornellUniversity,Geneva,NewYork,UnitedStatesofAmerica,5DepartmentofEntomology,UniversityofIllinoisatUrbana-Champaign,
Urbana,Illinois,UnitedStatesofAmerica
Abstract
Background:TheEuropeancornborer(ECB),Ostrinianubilalis(Hubner),existsastwoseparatesexpheromoneraces.ECB(Z)
femalesproducea97:3blendofZ11-andE11-tetradecenylacetatewhereasECB(E)femalesproduceanopposite1:99ratio
oftheZandEisomers.Malesofeachracerespondspecificallytotheirconspecificfemale’sblend.Acloselyrelatedspecies,
theAsiancornborer (ACB), O.furnacalis,usesa3:2blendofZ12-andE12-tetradecenylacetate, andisbelievedtohave
evolved from anECB-like ancestor. To further knowledge of the molecular mechanisms of pheromone detection andits
evolutionamongcloselyrelatedspeciesweidentifiedandcharacterizedsexpheromonereceptorsfromECB(Z).
Methodology:Homology-dependent(degeneratePCRprimersdesignedtoconservedaminoacidmotifs)andhomology-
independent(pyrophosphatesequencingofantennalcDNA)approacheswereusedtoidentifycandidatesexpheromone
transcripts. Expression in male and female antennae was assayed by quantitative real-time PCR. Two-electrode voltage
clampelectrophysiologywasusedtofunctionallycharacterizecandidatereceptorsexpressedinXenopusoocytes.
Conclusion:Wecharacterizedfivesexpheromonereceptors,OnOrs1and3–6.Theirtranscripts
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