Shedding Light on Filovirus Infection with High-Content Imaging 英文参考文献.docVIP

Viruses 2012, 4, 1354-1371; doi:10.3390/v4081354 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Review Shedding Light on Filovirus Infection with High-Content Imaging Gianluca Pegoraro 1,2, Sina Bavari 1 and Rekha G. Panchal 1,* 1 United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD 21702, USA; E-Mails: gianluca.pegoraro.ctr@ (G.P.); sina.bavari@ (S.B.) 2 PerkinElmer, 940 Winter Street, Waltham, MA 02451, USA * Author to whom correspondence should be addressed; E-Mail: rekha.panchal@; Tel.: +1-301-619-4985; Fax: +1-301-619-2348. Received: 6 July 2012; in revised form: 9 August 2012 / Accepted: 9 August 2012 / Published: 23 August 2012 Abstract: Microscopy has been instrumental in the discovery and characterization of microorganisms. Major advances in high-throughput fluorescence microscopy and automated, high-content image analysis tools are paving the way to the systematic and quantitative study of the molecular properties of cellular systems, both at the population and at the single-cell level. High-Content Imaging (HCI) has been used to characterize host-virus interactions in genome-wide reverse genetic screens and to identify novel cellular factors implicated in the binding, entry, replication and egress of several pathogenic viruses. Here we present an overview of the most significant applications of HCI in the context of the cell biology of filovirus infection. HCI assays have been recently implemented to quantitatively study filoviruses in cell culture, employing either infectious viruses in a BSL-4 environment or surrogate genetic systems in a BSL-2 environment. These assays are becoming instrumental for small molecule and siRNA screens aimed at the discovery of both cellular therapeutic targets and of compounds with anti-viral properties. We discuss