Simultaneous Detection and Differentiation of Human Papillomavirus Genotypes 6, 11, 16 and 18 by AllGlo Quadruplex Quantitative PCR 英文参考文献.docVIP

下载本文档

5
0
约3.49万字
约 7页
2017-05-13 发布于上海
举报
版权申诉

Simultaneous Detection and Differentiation of Human Papillomavirus Genotypes 6, 11, 16 and 18 by AllGlo Quadruplex Quantitative PCR 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Simultaneous Detection and Differentiation of Human Papillomavirus Genotypes 6, 11, 16 and 18 by AllGlo Quadruplex Quantitative PCR 英文参考文献

SimultaneousDetectionandDifferentiationofHuman PapillomavirusGenotypes6,11,16and18byAllGlo QuadruplexQuantitativePCR DaojunYu1,2,YuChen1,ShenghaiWu2,BaohongWang1,Yi-WeiTang3,LanjuanLi1* 1StateKeyLaboratoryfortheDiagnosisandTreatmentofInfectiousDiseases,TheFirstAffiliatedHospital,SchoolofMedicine,ZhejiangUniversity,Hangzhou,China, 2DepartmentofClinicalLaboratories,HangzhouFirstPeople’sHospital,Hangzhou,China,3ClinicalMicrobiologyService,MemorialSloan-KetteringCancerCenter,New York,NewYork,UnitedStatesofAmerica Abstract Background:Humanpapillomaviruses(HPV)areclassifiedintohigh-riskHPVandlow-riskHPV.Themostcommonhigh-risk HPVtypesincervicalcancerareHPV16and18,andthemostcommonlow-risktypescausinggenitalwartsareHPV6and HPV11.Inthisstudy,applyingnovelAllGlofluorescentprobes,weestablishedaquadruplexquantitativePCRmethodto simultaneouslydetectanddifferentiateHPV6,11,16and18inasingletube. Methods:Thespecificity,thesensitivity,thedetectionlimit,thereproducibilityandthestandardcurveofthismethodwere examined.Finally,clinicalsamplesthathadbeentestedpreviouslybyTaqManPCRandHPVGenoArray(GA)testwereused toverifytheaccuracyandsensitivityofthemethod. Results:Theassayhasasensitivityof101to102copies/testandalineardetectionrangefrom101to108copies/test.The mean amplification efficiencies for HPV 6, 11, 16, and 18 were 0.97, 1.10, 0.93 and 1.20, respectively, and the mean correlationcoefficient(r2)ofeachstandardcurvewasabove0.99forplasmidtemplatesrangingfrom103to107copies/test. There was 100% agreement between the AllGlo quadruplex quantitative PCR, HPV GA test and TaqMan uniplex qPCR methods. Conclusions:AllGloquadruplexquantitativePCRinasingletubehastheadvantagesofrelativelyhighthroughput,good reproducibility,highsensitivity,highspecificity,andawidelinearrangeofdetection.Theconvenientsingletubeformat makes this assay a powerful tool for the studies of mixed infections by multiple pathogens, viral typing and viral load quantification. Citation:YuD,ChenY,WuS,WangB,TangY-W,etal.(2012)Si