Simultaneous Detection of Multiple Fish Pathogens Using a Naked-Eye Readable DNA Microarray 英文参考文献.docVIP

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Simultaneous Detection of Multiple Fish Pathogens Using a Naked-Eye Readable DNA Microarray 英文参考文献.doc

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Simultaneous Detection of Multiple Fish Pathogens Using a Naked-Eye Readable DNA Microarray 英文参考文献

Sensors 2012, 12, 2710-2728; doi:10.3390/s120302710 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Simultaneous Detection of Multiple Fish Pathogens Using a Naked-Eye Readable DNA Microarray Chin-I Chang 1,*,?, Pei-Hsin Hung 2,?, Chia-Che Wu 1, Ta Chih Cheng 3, Jyh-Ming Tsai 4, King-Jung Lin 1 and Chung-Yen Lin 5,6,7 1 Aquaculture Division, Fisheries Research Institute, Ministry of Agriculture, 199 Hou-Ih Road, Keelung 20246, Taiwan; E-Mails: ccwu02@.tw (C.-C.W.); kjlin@.tw (K.-J.L.) 2 Department of Aquaculture, College of Life Sciences, National Taiwan Ocean University, Pei-Ning Road, Keelung 20224, Taiwan; E-Mail: peihsin.hung@ 3 Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, 1 Hseuh-Fu Rd., Nei-Pu Hsiang, Pingtung 91201, Taiwan; E-Mail: cheng.tachih@ 4 Department of Marine Biotechnology, National Kaohsiung Marine University, 142 Hai-Chuan Road, Kaohsiung 81157, Taiwan; E-Mail: jmtsai@.tw 5 Institute of Information Science, Academia Sinica, No. 128 Academia Rd., Sec. 2, Taipei 115, Taiwan; E-Mail: cylin@.tw 6 Institute of Fisheries Science, College of Life Science, National Taiwan University, No. 1, Roosevelt Rd., Sec 4, Taipei 10617, Taiwan 7 Division of Biostatistics and Bioinformatics, Institute of Population Health Sciences, National Health Research Institutes, No. 35, Keyan Rd., Zhunan 350, Taiwan ? These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: cichang@.tw; Tel.: +886-2-2462-2101 ext. 2808; Fax: +886-2-2462-8138. Received: 6 February 2012; in revised form: 20 February 2012 / Accepted: 27 February 2012 / Published: 29 February 2012 Abstract: We coupled 16S rDNA PCR and DNA hybridization technology to construct a microarray for simultaneous detection and discrimination of eight fish pathogens (Aeromonas hydrophila, Edwardsiella tarda, Flavobacterium columnare, Lactococcus garv