Simultaneous RNA-Seq Analysis of a Mixed Transcriptome of Rice and Blast Fungus Interaction 英文参考文献.docVIP

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Simultaneous RNA-Seq Analysis of a Mixed Transcriptome of Rice and Blast Fungus Interaction 英文参考文献.doc

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Simultaneous RNA-Seq Analysis of a Mixed Transcriptome of Rice and Blast Fungus Interaction 英文参考文献

SimultaneousRNA-SeqAnalysisofaMixed TranscriptomeofRiceandBlastFungusInteraction YoshihiroKawahara1,YoukoOono1,HiroyukiKanamori1,TakashiMatsumoto1,TakeshiItoh1*, EiichiMinami2 1Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan, 2Genetically Modified Organism Research Center, National InstituteofAgrobiologicalSciences,Tsukuba,Ibaraki,Japan Abstract Afilamentousfungus,Magnaportheoryzae,isacausalagentofriceblastdisease,whichisoneofthemostseriousdiseases affectingcultivatedrice,Oryzasativa.However,themolecularmechanismsunderlyingbothricedefenseandfungalattack arenotyetfullyunderstood.Extensivepaststudieshavecharacterizedmanyinfection-responsivegenesinthepathogen and host plant, separately. To understand the plant-pathogen interaction comprehensively, it is valuable tomonitor the geneexpressionprofilesofbothinteractingorganismssimultaneouslyinthesameinfectedplanttissue.Althoughthehost- pathogen interaction during the initial infection stage is important for the establishment of infection, the detection of fungal gene expression in infected leaves at the stage has been difficult because very few numbers of fungal cells are present.UsingtheemergingRNA-Seqtechnique,whichhasawidedynamicrangeforexpressionanalyses,weanalyzedthe mixedtranscriptomeofriceandblastfungusininfectedleavesat24hourspost-inoculation,whichisthepointwhenthe primaryinfectionhyphaepenetrateleafepidermalcells.Wedemonstratedthatourmethoddetectedthegeneexpression ofboththehostplantandpathogensimultaneouslyinthesameinfectedleafbladesinnaturalinfectionconditionswithout any artificial treatments. The upregulation of 240 fungal transcripts encoding putative secreted proteins was observed, suggesting that these candidates of fungal effector genes may play important roles in initial infection processes. The upregulationoftranscriptsencodingglycosylhydrolases,cutinasesandLysMdomain-containingproteinswereobservedin theblastfungus,whereaspathogenesis-relatedandphytoa