SLC37A1 and SLC37A2 Are Phosphate-Linked, Glucose-6-Phosphate Antiporters 英文参考文献.docVIP

SLC37A1andSLC37A2ArePhosphate-Linked, Glucose-6-PhosphateAntiporters Chi-JiunnPan.,Shih-YinChen.,HyunSikJun,SuRuLin,BrianC.Mansfield,JaniceY.Chou* Section on Cellular Differentiation, Program in Developmental Endocrinology and Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development,NationalInstitutesofHealth,Bethesda,Maryland,UnitedStatesofAmerica Abstract Bloodglucosehomeostasisbetweenmealsdependsuponproductionofglucosewithintheendoplasmicreticulum(ER)of theliverandkidneybyhydrolysisofglucose-6-phosphate(G6P)intoglucoseandphosphate(Pi).Thisreactiondependson couplingtheG6Ptransporter(G6PT)withglucose-6-phosphatase-a(G6Pase-a).OnlyoneG6PT,alsoknownasSLC37A4,has beencharacterized,anditactsasaPi-linkedG6Pantiporter.TheotherthreeSLC37familymembers,predictedtobesugar- phosphate:Pi exchangers, have not been characterized functionally. Using reconstituted proteoliposomes, we examine the antiporter activity of the other SLC37 members along with their ability to couple with G6Pase-a. G6PT- and mock- proteoliposomes are used as positive and negative controls, respectively. We show that SLC37A1 and SLC37A2 are ER- associated,Pi-linkedantiporters,thatcantransportG6P.UnlikeG6PT,neitherissensitivetochlorogenicacid,acompetitive inhibitor of physiological ER G6P transport, and neither couples to G6Pase-a. We conclude that three of the four SLC37 familymembersarefunctionalsugar-phosphateantiporters.However,onlyG6PT/SLC37A4matchesthecharacteristics of the physiological ER G6P transporter, suggesting the other SLC37 proteins have roles independent of blood glucose homeostasis. Citation: Pan C-J,Chen S-Y, Jun HS, Lin SR, Mansfield BC, et al. (2011) SLC37A1and SLC37A2 Are Phosphate-Linked, Glucose-6-Phosphate Antiporters. PLoS ONE6(9):e23157.doi:10.1371/journal.pone.0023157 Editor:DanZilberstein,Technion-IsraelInstituteofTechnologyHaifa32000Israel,Israel ReceivedMay6,2011;AcceptedJuly7,2011;PublishedSeptember20,2011 Thisisanopen-accessarticle,fr