Solar Ultraviolet-B Radiation Increases Phenolic Content and Ferric Reducing Antioxidant Power in Avena sativa 英文参考文献.docVIP

Molecules 2007, 12, 1220-1232 molecules ISSN 1420-3049 ? 2007 by MDPI /molecules Full Paper Solar Ultraviolet-B Radiation Increases Phenolic Content and Ferric Reducing Antioxidant Power in Avena sativa Christopher T. Ruhland *, Mitchell J. Fogal, Christopher R. Buyarski and Matthew A. Krna Department of Biological Sciences, TS-242 Trafton Sciences Center, Minnesota State University, Mankato, MN 56001, USA; E-mail: christopher.ruhland@ *Author to whom correspondence should be addressed. Received: 1 May 2007 / Accepted: 26 June 2007 / Published: 29 June 2007 Abstract: We examined the influence of solar ultraviolet-B radiation (UV-B; 280-320 nm) on the maximum photochemical efficiency of photosystem II (Fv/Fm), bulk-soluble phenolic concentrations, ferric-reducing antioxidant power (FRAP) and growth of Avena sativa. Treatments involved placing filters on frames over potted plants that reduced levels of biologically effective UV-B by either 71% (reduced UV-B) or by 19% (near-ambient UV-B) over the 52 day experiment (04 July - 25 August 2002). Plants growing under near- ambient UV-B had 38% less total biomass than those under reduced UV-B. The reduction in biomass was mainly the result of a 24% lower leaf elongation rate, resulting in shorter leaves and less total leaf area than plants under reduced UV-B. In addition, plants growing under near-ambient UV-B had up to 17% lower Fv/Fm values early in the experiment, and this effect declined with plant age. Concentrations of bulk-soluble phenolics and FRAP values were 17 and 24% higher under near-ambient UV-B than under reduced UV-B, respectively. There was a positive relationship between bulk-soluble phenolic concentrations and FRAP values. There were no UV-B effects on concentrations of carotenoids (carotenes + xanthophylls). Keywords: antioxidant activity, flavonoids, FRAP, total phenols, UV-B  Molecules 2007, 12 1221 Intro