Staphylococcal peptidoglycans induce arthritis 英文参考文献.docVIP

Available online /content/3/6/375 Research article Staphylococcal peptidoglycans induce arthritis Zai-Qing Liu*, Guo-Min Deng*, Simon Foster? and Andrej Tarkowski* *Department of Rheumatology, University of G?teborg, G?teborg, Sweden ?Department of Molecular Biology and Biotechnology, University of Sheffield, UK Correspondence: Zai-Qing Liu, Department of Rheumatology, University of G?teberg, Guldhedsgatan 10A, 41346 G?teberg, Sweden. Tel: +46 31 3426452; e-mail: zai-qing@rheuma.gu.se Received: 14 June 2001 Arthritis Res 2001, 3:375-380 Revisions requested: 7 August 2001 Revisions received: 15 August 2001 Accepted: 24 August 2001 This article may contain supplementary data which can only be found online at /content/3/6/375 ? 2001 Liu et al, licensee BioMed Central Ltd Published: 17 September 2001 (Print ISSN 1465-9905; Online ISSN 1465-9913) Abstract Staphylococcus aureus is one of the most important pathogens in septic arthritis. To analyse the arthritogenic properties of staphylococcal peptidoglycan (PGN), highly purified PGN from S. aureus was intra-articularly injected into murine joints. The results demonstrate that PGN will trigger arthritis in a dose-dependent manner. A single injection of this compound leads to massive infiltration of predominantly macrophages and polymorphonuclear cells with occasional signs of cartilage and/or bone destruction, lasting for at least 14 days. Further studies showed that this condition is mediated by the combined impact of acquired and innate immune systems. Our results indicate that PGN exerts a central role in joint inflammation triggered by S. aureus. Keywords: arthritis, peptidoglycan, Staphylococcus aureus Introduction Peptidoglycan (PGN) is the major component of the cell wall of Gram-positive bacteria and is composed of long sugar chains of alternating N-acetylglucosamine and N- acetylmuramic acid residues, which are highly cross-linked via peptide side chain