Structural Characterisation by ESI-MS of Feruloylated Arabino-oligosaccharides Synthesised by Chemoenzymatic Esterification 英文参考文献.docVIP

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Structural Characterisation by ESI-MS of Feruloylated Arabino-oligosaccharides Synthesised by Chemoenzymatic Esterification 英文参考文献.doc

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Structural Characterisation by ESI-MS of Feruloylated Arabino-oligosaccharides Synthesised by Chemoenzymatic Esterification 英文参考文献

Molecules 2007, 12, 1367-1375 molecules ISSN 1420-3049 ? 2007 by MDPI /molecules Full Paper Structural Characterisation by ESI-MS of Feruloylated Arabino-oligosaccharides Synthesised by Chemoenzymatic Esterification Christina Vafiadi 1, Evangelos Topakas 1, Edwin J. Bakx 2, Henk A. Schols 2 and Paul Christakopoulos 1,* 1 Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700, Athens Greece 2 Wageningen University, Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry,P.O. Box 8129, 6700 EV Wageningen, The Netherlands * Author to whom correspondence should be addressed; Tel: +30-210-7723231; fax: +30-210-7723163; e-mail: hristako@orfeas.chemeng.ntua.gr Received: 12 June 2007; in revised form: 3 July 2007 / Accepted: 3 July 2007 / Published: 9 July 2007 Abstract: The chemoenzymatic synthesis of feruloylated arabino-oligosaccharides has been achieved, using a feruloyl esterase type C from Sporotrichum thermophile (StFaeC). The structure of the feruloylated products was confirmed by ESI-MSn. Keywords: Feruloyl esterase; Enzymatic transesterification; Feruloyl oligosaccharides; Detergentless microemulsions Introduction Feruloyl esterases (FAEs; E.C. 3) are the enzymes responsible for cleaving the ester-link between the main chain polysaccharides of xylans or pectins and monomeric or dimeric ferulic acid. These polysaccharides, which complex with polymers like lignin and cellulose, modulate the plant cell wall material. FAEs are classified into four functional sub-classes (A, B, C and D) according to their primary amino acid sequence identity, specificity for the hydrolysis of hydroxycinnamic acid methyl esters, ability to release 5,5′-diferulic acid from model and complex substrates and inducible plant cell Molecules 2007, 12 1368 wall materials [1]. Various hydroxycinnamic acids (ferulic, p-c