Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia 英文参考文献.docVIP

Wangetal.BMCPlantBiology2010,10:190 /1471-2229/10/190 RESEARCH ARTICLE OpenAccess TranspositionalreactivationoftheDart transposonfamilyinricelinesderivedfrom introgressivehybridizationwithZizanialatifolia NingningWang1,HongyanWang1,2,HuiWang1,DiZhang1,YingWu1,XiufangOu1,ShuangLiu1,ZhenyingDong1, BaoLiu1* Abstract Background:Itiswidelyrecognizedthatinterspecifichybridizationmayinduce“genomeshock”,andleadto geneticandepigeneticinstabilitiesintheresultanthybridsand/orbackcrossedintrogressants.Aprominent componentinvolvedinthegenomeshockisreactivationofcryptictransposableelements(TEs)inthehybrid genome,whichisoftenassociatedwithalterationintheelements’epigeneticmodificationslikecytosineDNA methylation.WehavepreviouslyreportedthatintrogressantsderivedfromhybridizationbetweenOryzasativa(rice) andZizanialatifoliamanifestedsubstantialmethylationre-patterningandrampantmobilizationoftwoTEs,acopia retrotransposonTos17andaMITEmPing.ItwasnotknownhoweverwhetherothertypesofTEshadalsobeen transpositionallyreactivatedintheseintrogressants,theirrelevancetoalterationincytosinemethylation,andtheir impactonexpressionofadjacentcellulargenes. Results:WedocumentinthisstudythattheDartTEfamilywastranspositionallyreactivatedfollowedby stabilizationinallthreestudiedintrogressants(RZ1,RZ2andRZ35)derivedfromintrogressivehybridization betweenrice(cv.Matsumae)andZ.latifolia,whiletheTEsremainedquiescentintherecipientricegenome. Transposon-display(TD)andsequencingverifiedtheelement’smobilityandmappedtheexcisionsandre- insertionstothericechromosomes.Methylation-sensitiveSouthernblottingshowedthattheDartTEswereheavily methylatedalongtheirentirelength,andmoderatealterationincytosinemethylationpatternsoccurredinthe introgressantsrelativetotheirriceparentalline.Real-timeqRT-PCRquantificationontherelativetranscript abundanceofsixsingle-copygenesflankingthenewlyexcisedorinsertedDart-relatedTEcopiesindicatedthat whereasmarkeddifferenceintheexpressionofallfourgenesinbothtissues(leafandroot)weredetected be