Two or more enteropathogens are associated with diarrhoea in Mexican children 英文参考文献.docVIP

Annals of Clinical Microbiology and Antimicrobials BioMedCentral Research Open Access Two or more enteropathogens are associated with diarrhoea in Mexican children Gloria Luz Paniagua, Eric Monroy, Octavio García-González, Javier Alonso, Erasmo Negrete and Sergio Vaca* Address: Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Avenida de Los Barrios. 1, Los Reyes Iztacala, Tlalnepantla, 54090, Estado de Mexico, Mexico Email: Gloria Luz Paniagua - mya@servidor.unam.mx; Eric Monroy - mopi@servidor.unam.mx; Octavio García- González - octaviopatricio@; Javier Alonso - alonsot1212@.mx; Erasmo Negrete - negretee@; Sergio Vaca* - vsergio@servidor.unam.mx * Corresponding author Published: 28 December 2007 Received: 1 October 2007 Accepted: 28 December 2007 Annals of Clinical Microbiology and Antimicrobials 2007, 6:17 doi:10.1186/1476-0711-6- 17 This article is available from: /content/6/1/17 ? 2007 Paniagua et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Diarrhoeal diseases constitute a major public health problem, particularly in the developing world, where the rate of mortality and morbidity is very high. The purpose of this study was to conduct a 2 years and 3 months study in order to determine the prevalence of five enteropathogen diarrheogenic agents in Mexico City. Methods: Faecal samples were obtained from 300 Mexican children diagnosed as positive for diarrhoea, aged 2 to 12 years old, and from 80 children matched for age but with no symptoms of the disease (control group). Two multiplex PCR were used to detect Escherichia coli, Salmonella spp., and Shigella spp. In addition, the two protozoan parasites Entamoeba histolytic