Use of Spectroscopic, Zeta Potential and Molecular Dynamic Techniques to Study the Interaction between Human Holo-Transferrin and Two Antagonist Drugs Comparison of Binary and Ternary Systems 英文参考文献.docVIP

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Use of Spectroscopic, Zeta Potential and Molecular Dynamic Techniques to Study the Interaction between Human Holo-Transferrin and Two Antagonist Drugs Comparison of Binary and Ternary Systems 英文参考文献.doc

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Use of Spectroscopic, Zeta Potential and Molecular Dynamic Techniques to Study the Interaction between Human Holo-Transferrin and Two Antagonist Drugs Comparison of Binary and Ternary Systems 英文参考文献

Molecules 2012, 17, 3114-3147; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Use of Spectroscopic, Zeta Potential and Molecular Dynamic Techniques to Study the Interaction between Human Holo-Transferrin and Two Antagonist Drugs: Comparison of Binary and Ternary Systems Mona Kabiri 1, Zeinab Amiri-Tehranizadeh 2, Ali Baratian 2, Mohammad Reza Saberi 2 and Jamshidkhan Chamani 1,* 1 Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad 9175687119, Iran; E-Mail: mona.kabiri@ 2 Medical Chemistry Department, School of Pharmacy, Mashhad University of Medical Science, Mashhad 9175687119, Iran; E-Mails: zeinab.amiri86@ (Z.A.-T.); baratian841@mums.ac.ir (A.B.); saberimr@mums.ac.ir (M.R.S.) * Author to whom correspondence should be addressed; E-Mail: chamani@ibb.ut.ac.ir; chamani_j@; Tel.: +98-511-843-7107; Fax: +98-511-842-4020. Received: 1 December 2011; in revised form: 24 February 2012 / Accepted: 28 February 2012 / Published: 12 March 2012 Abstract: For the first time, the binding of ropinirole hydrochloride (ROP) and aspirin (ASA) to human holo-transferrin (hTf) has been investigated by spectroscopic approaches (fluorescence quenching, synchronous fluorescence, time-resolved fluorescence, three-dimensional fluorescence, UV-vis absorption, circular dichroism, resonance light scattering), as well as zeta potential and molecular modeling techniques, under simulated physiological conditions. Fluorescence analysis was used to estimate the effect of the ROP and ASA drugs on the fluorescence of hTf as well as to define the binding and quenching properties of binary and ternary complexes. The synchronized fluorescence and three-dimensional fluorescence spectra demonstrated some micro-environmental and conformational changes around the Trp and Tyr residues with a faint red shift. Thermodynamic analysis displ