Using Monoclonal Antibody to Determine Lead Ions with a Localized Surface Plasmon Resonance Fiber-optic Biosensor 英文参考文献.docVIP

Sensors 2008, 8, 582-593 sensors ISSN 1424-8220 ? 2008 by MDPI /sensors Full Research Paper Using Monoclonal Antibody to Determine Lead Ions with a Localized Surface Plasmon Resonance Fiber-optic Biosensor Tsao-Jen Lin * and Mon-Fu Chung Department of Chemical Engineering, National Chung-Cheng University, Chia-Yi 621, Taiwan * Author to whom correspondence should be addressed. E-mail: chmtjl@.tw; Tel: 886-5-272044 ext 33405 Received: 4 October 2007 / Accepted: 24 January 2008 / Published: 25 January 2008 Abstract: A novel reflection-based localized surface plasmon resonance (LSPR) fiber- optic probe has been developed to determine the heavy metal lead ion concentration. Monoclonal antibody as the detecting probe containing massive amino groups to capture Pb(II)-chelate complexes was immobilized onto gold nanoparticle-modified optical fiber (NMAuOF). The optimal immobilizing conditions of monoclonal antibody on to the NMAuOF are 189 μg/mL in pH7.4 PBS for 2 h at 25°C. The absorbability of the functionalized NMAuOF sensor increases to 12.2 % upon changing the Pb(II)-EDTA level from 10 to 100 ppb with a detection limit of 0.27 ppb. The sensor retains 92.7 % of its original activity and gives reproducible results after storage in 5% D-(+)-Trehalose dehydrate solution at 4°C for 35 days. In conclusion, the monoclonal antibody- functionalized NMAuOF sensor shows a promising result for determining the concentration of Pb(II) with high sensitivity. Keywords: localized surface plasmon resonance, biosensor, gold nanoparticle, monoclonal antibody, lead-chelate complex. 1. Introduction Lead is one of the most widely used heavy metals and has a large number of industrial applications, including battery manufacture, paint, gasoline, alloys, radiation shielding, tank lining and piping. Among these, lead in paint and gasoline together accounts