UTILLdb, a Pisum sativum in silico forward and reverse genetics tool 英文参考文献.docVIP

Open Access Method 2008DalmaisetVoal.ume 9, Issue 2, Article R43 UTILLdb, a Pisum sativum in silico forward and reverse genetics tool ¤ ¤ ¤ Marion Dalmais *, Julien Schmidt *, Christine Le Signor ?, Francoise Moussy?, Judith Burstin?, Vincent Savois?, Gregoire Aubert?, Veronique Brunaud*, Yannick de Oliveira*, Cecile Guichard*, Richard Thompson? and Abdelhafid Bendahmane* Addresses: *Unité de Recherche en Génomique Végétale, UMR INRA-CNRS, Rue Gaston Crémieux, 91057 Evry Cedex, France. ?INRA, Unite Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses (INRA-ENESAD), Domaine dEpoisses, 21110 Bretenières, France. ¤ These authors contributed equally to this work. Correspondence: Abdelhafid Bendahmane. Email: bendahm@evry.inra.fr Published: 26 February 2008 Received: 29 November 2007 Revised: 17 January 2008 Accepted: 26 February 2008 Genome Biology 2008, 9:R43 (doi:10.1186/gb-2008-9-2-r43) The electronic version of this article is the complete one and can be found online at /2008/9/2/R43 ? 2008 Dalmais et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. PealaptiogeneticsUn.TI/LpLdbdatabaseis a database of phenotypic and sequence information on mutant genes from a reference Pisum sativum EMS-mutant popu- Abstract The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the B