Sedum Panax flavonoid content analysis of conditions and.docVIP

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2017-05-17 发布于浙江
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Sedum Panax flavonoid content analysis of conditions and.doc

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Sedum Panax flavonoid content analysis of conditions and

Sedum Panax flavonoid content analysis of conditions and [Abstract] Objective: To study the different extraction solvents on the Sedum Panax content of total flavonoids and total flavonoids under the optimum conditions of the content. Methods: hydrochloric acid magnesium method and lead acetate method of total flavonoids Sedum Panax check knowledge and use of different solvents on Sedum notoginseng was extracted flavonoids to Determination of total flavonoids, rutin as the standard to 10% Al (NO3) 3 5% NaNO2 as chromogenic agent were measured at 508nm wavelength. Results: The examination tests knowledge proof of Sedum notoginseng contains flavonoids, and the best extraction solvent is 80% ethanol (P lt;0.05). In this extraction condition, the measured Sedum Panax The content of total flavonoids 18.39mg g-1, RSD was 2.2% (n = 5). flavonoids linear range was 15.20 ~ 45.60g mL-1 (r = 0.999 7), the average recovery was 95.74 %, RSD = 0.98% (n = 6). Conclusion: Flavonoids Sedum notoginseng the best extraction solvent is 80% ethanol. The method is accurate, reliable, reproducible results are stable for three Sedum Analysis of Flavonoids in July. [Keywords:] Sedum notoginseng; flavonoids; spectrophotometry [ABSTRACT] Objective: To study the extracting conditions and determination for total flavonoids in Se dum Aizoon L. with different solvent. Methods: To determine the flavonoids in Sedum Aizoon L. by HCl Mg and Pb (AC) 2 reactions. Rutin was taken as standard, the colour developing agent was the mixed solution of 10% Al (NO3) 3 ~ 5% NaNO2.The total flavonoids in Sedum Aizoon L. were analyzed by spectrophotometry at 508nm, and series of extracting solvents were used to total flavonoids. Results: There were flavonoids in Sedum Aizoon L. and the best extracting solvent of all was 80% ethanol.Calibration curve was constructed in the range of 15.20 ~ 45.60g mL-1 (r = 0.999 7) for total flavonoids.The average recovery was 95.74% and RSD of the methods was 0.98% (n =