Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts.docVIP

Molecules 2012, 17, 2518-2528; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Effects of Different Extraction Methods and Conditions on the Phenolic Composition of Mate Tea Extracts Nevena Grujic 1,*, Zika Lepojevic 2, Branislava Srdjenovic 1, Jelena Vladic 1 and Jan Sudji 3 1 Department of Pharmacy, Medical Faculty, University of Novi Sad, Serbia; E-Mails: srdjbr@ (B.S.); vladicjelena@ (J.V.) 2 Department of Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Serbia; E-Mail: lepojevic@tf.uns.ac.rs 3 The Institute for the Health Protection of Workers Novi Sad, Serbia; E-Mail: sudy@ * Author to whom correspondence should be addressed; E-Mail: nevenagrujic@; Tel.: +381-21-422-760; Fax: +381-21-422-760. Received: 12 January 2012; in revised form: 24 February 2012 / Accepted: 27 February 2012 / Published: 2 March 2012 Abstract: A simple and rapid HPLC method for determination of chlorogenic acid (5-O-caffeoylquinic acid) in mate tea extracts was developed and validated. The chromatography used isocratic elution with a mobile phase of aqueous 1.5% acetic acid-methanol (85:15, v/v). The flow rate was 0.8 mL/min and detection by UV at 325 nm. The method showed good selectivity, accuracy, repeatability and robustness, with detection limit of 0.26 mg/L and recovery of 97.76%. The developed method was applied for the determination of chlorogenic acid in mate tea extracts obtained by ethanol extraction and liquid carbon dioxide extraction with ethanol as co-solvent. Different ethanol concentrations were used (40, 50 and 60%, v/v) and liquid CO2 extraction was performed at different pressures (50 and 100 bar) and constant temperature (27 ± 1 °C). Significant influence of extraction methods, conditions and solvent polarity on chlorogenic acid content, antioxidant activity and total phenolic and flavonoid co