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Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR.doc

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Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR

BMC Plant Biology BioMedCentral Research article Open Access Exploring valid reference genes for gene expression studies in Brachypodium distachyon by real-time PCR Shin-Young Hong?1, Pil Joon Seo?1, Moon-Sik Yang2, Fengning Xiang3 and Chung-Mo Park*1 Address: 1Department of Chemistry, Seoul National University, Seoul, 151-742, Korea , 2Division of Biological Sciences and the Research Institute for Bioactive Materials, Chonbuk National University, Jeonju, 561-756, Korea and 3School of Life Sciences, Shandong University, Jinan 250100, Shandong, PR China Email: Shin-Young Hong - cutesy77@yahoo.co.kr; Pil Joon Seo - dualnt83@snu.ac.kr; Moon-Sik Yang - mskyang@chonbuk.ac.kr; Fengning Xiang - xfn0990@; Chung-Mo Park* - cmpark@snu.ac.kr * Corresponding author ?Equal contributors Published: 7 November 2008 Received: 29 May 2008 Accepted: 7 November 2008 BMC Plant Biology 2008, 8:112 doi:10.1186/1471-2229-8-112 This article is available from: /1471-2229/8/112 ? 2008 Hong et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: The wild grass species Brachypodium distachyon (Brachypodium hereafter) is emerging as a new model system for grass crop genomics research and biofuel grass biology. A draft nuclear genome sequence is expected to be publicly available in the near future; an explosion of gene expression studies will undoubtedly follow. Therefore, stable reference genes are necessary to normalize the gene expression data. Results: A systematic exploration of suitable reference genes in Brachypodium is presented here. Nine reference gene candidates were chosen, and their gene sequences were obtained from the Brachypodium expressed sequence tag (EST) databases. Their expression levels