Genome scale transcriptome analysis of shoot organogenesis in Populus.docVIP

BMC Plant Biology BioMedCentral Research article Open Access Genome scale transcriptome analysis of shoot organogenesis in Populus Yanghuan Bao1, Palitha Dharmawardhana1, Todd C Mockler2,3 and Steven H Strauss*1,3 Address: 1Department of Forest Ecosystems and Society, Oregon State University, Corvallis, Oregon 97331-5752, USA, 2Department of Botany and Plant Pathology, Cordley Hall 2082, Oregon State University, Corvallis, Oregon 97331-2902, USA and 3Center for Genome Research and Biocomputing, Oregon State University, Corvallis, Oregon 97331-7303, USA Email: Yanghuan Bao - yangh.bao@; Palitha Dharmawardhana - palitha@; Todd C Mockler - tmockler@; Steven H Strauss* - steve.strauss@ * Corresponding author Published: 17 November 2009 Received: 12 January 2009 Accepted: 17 November 2009 BMC Plant Biology 2009, 9:132 doi:10.1186/1471-2229-9-132 This article is available from: /1471-2229/9/132 ? 2009 Bao et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Our aim is to improve knowledge of gene regulatory circuits important to dedifferentiation, redifferentiation, and adventitious meristem organization during in vitro regeneration of plants. Regeneration of transgenic cells remains a major obstacle to research and commercial deployment of most taxa of transgenic plants, and woody species are particularly recalcitrant. The model woody species Populus, due to its genome sequence and amenability to in vitro manipulation, is an excellent species for study in this area. The genes recognized may help to guide the development of new tools for improving the efficiency of plant regeneration and transformation. Results: We analyzed gene expression during poplar in vitro d