流式细胞flowcytometry.pptVIP

画门（Gating） Whole blood light scatter Gate on lymphocytes (light scatter) Assess T-cell population (fluorescence) Sample Sample/singlets Sample/singlets/CD3/CD4+ Sample/singlets/CD3 Software Sources Flow cytometer manufacturers Commercial software sources De Novo Software ? FCS Express Management Sciences Associates ? MacLAS WinLAS Phoenix Flow Systems ? MultiCycle AV, Win-FCM, MultiTime , etc. Ray Hicks ? FCSPress (Macintosh) Tree Star, Inc. ? FloJo Verity Software House ? Wi