基因诊断与基因治疗概论课件GeneDT08-31.pptVIP

Disease is related not only to the structure of gene but also to the transcription or translation level Gene diagnosis may put in practice by: 1. DNA diagnosis 2. RNA diagnosis 3. Fluorescence in situ Hybridization (FISH) 4. Gene chip Disease is related not only to the structure of gene but also to the transcription or translation level Gene diagnosis may put in practice by: 1. DNA diagnosis 2. RNA diagnosis 3. Fluorescence in situ Hybridization (FISH) 4. Gene chip The abnormal structure of gene may be detected at DNA level directly. If the pathogenic gene or its structure and the details of mutation is uncertain, it is necessary to judge the existence of pathogenic gene indirectly by means of genetic markers. Such means is called Linkage analyses. The infectious disease may be diagnosed by detecting the exogenous DNA of pathogen. The abnormal structure of gene may be detected at DNA level directly. If the pathogenic gene or its structure and the details of mutation is uncertain, it is necessary to judge the existence of pathogenic gene indirectly by means of genetic markers. Such means is called Linkage analyses. The infectious disease may be diagnosed by detecting the exogenous DNA of pathogen. The abnormal structure of gene may be detected at DNA level directly. If the pathogenic gene or its structure and the details of mutation is uncertain, it is necessary to judge the existence of pathogenic gene indirectly by means of genetic markers. Such means is called Linkage analyses. The infectious disease may be diagnosed by detecting the exogenous DNA of pathogen. （1）Direct detection of gene mutation To judge the existence of known mutation. Be applicable to cases in which the pathogenic gene is certain and its sequence is complete or partial known.