奶牛粪便酶解混合菌群的构建及产酶条件的研究.pdfVIP

φ@怠曹(i 4l 2012,28(11):81-85 Chinese Agricultural Science Bulletin 奶牛粪便酶解混合菌群的构建及产酶条件的研究 杨为1 ，李静IZ ，徐静静1 ，周佳2 ，屈建强‘ (西南大学资源环境学院，重庆4∞716;重庆水利电力职业技术学院，重庆4021ω: 3 重庆市榆北区暨华中学，重庆401120户重庆市城市建设投资集团(有限〉公司〉 摘要:为了确立酶解混合菌群的最佳培养条件，达到最好的降解放果，有效利用奶牛粪便。以筛选的几 株菌株构建酶解混合菌群并研究其产酶条件。结果表明:F3-F9 组合的纤维素降解率达到44.02% ，比单 一菌株提高了 12.47%。温度、培养时间、起始pH值、培养基菌液比、添加款皮和氨源等因素都会对产酶 有较大影响。在培养基中添加0.1%尿素，固液比为 1:2 ，接种量为 5%，起始pH7，培养基中添加0.1%的 尿素和4肝b的敖皮，温度为24吧，培养3 天时F3-F9 混合菌株滤纸酶活和CMC酶活分别达到相对较高的 值，分别为 114.25 U和1617.92 U。 关键询:奶牛粪便;混合菌群;构建;产酶条件 申图分类号:X7;S2 文献标志码:A 论文编号:2011-3687 Construction of Mixed Flora for Enzymatic Hydrolysfs of Dalry Manure and Study of Fermentation Conditions l 3 Yang Li飞Li Jing ,2, Xu Jingji晖， Zhou Jia , Qu Jianqian矿 (Resource and Environment College. SouthweJt UniverJ坊， Chongqi唱4∞716; C加咱qing Water ReJources and Ele甜， icEi哺ineering College. Chongqi吨4021ω; lChongqing yi凶eiJihI.ωSclu.时， Cho吨;qing 401120; Cho咱l侨ng City COnJtructÍDn InveJtmω(命。叩，) Co.，úd叶Cho吨φ吨崎21ω) Abs衍act: For the pu甲ose of saccharification of dairy manure. Screening of several strains to construct strains of bacteria and ωstudy its e缸ymatic mixed conditions for enz归ne production. The results showed that the cellulose degradation rate of the F3-F9 pair accounted for 44.02% , which increased by 12.47%. Such 皿 temperature , cultivation time , initial pH , S几of medium, and addition of bran 嗣well 嗣 nitrogen source etc had significant impact on cellulase productivity. compared wi也 single strain fermentation. 0.1 % urea w皿 addedto the medium, S血.w，皿 1:2 and the inoculum