普通油茶花粉形态及花粉管活体萌发的研究.pdfVIP

2011 年6 月 中国油料作物学报 2011 ,33(3) :242 - 246 Chinese journal of oil crop sciences 普通油茶花粉形态及花粉管活体萌发的研究 李春林I 气姚小华1· ，杨水平2 ，任华东l ，曹永庆1 (1.中国林科院亚热带林业研究所，浙江富阳 3114∞;2. 西南大学资源环境学院，重庆4∞716) 摘要:利用扫描电子显微镜观察了3 个普通油茶优良品系的花粉形态，并利用荧光显微镜观察普通油茶杂交 授粉后不同时间段花粉管的生长状况，以探讨普通汹茶在山茶科中系统发育的地位以及花粉管在授粉后的动态发 育过程。结果表明，普通油茶花粉形态一般为椭球或近球形，大小中等，具三沟，外壁纹饰为脑纹或桔皮状，符合山 茶属花粉的种属特征，在山茶科系统发育中为较原始的类群:杂交授粉后O. 拙，花粉管开始萌发，8h 花粉管生长端 可达花柱中部，24h 穿过花柱基部进入子房，约48h 即可完成受精过程;花粉萌发试验表明活体授粉的花粉萌发及 生长量显著高于离体培养花扮。 关键词:普通油茶;花粉形态;花粉管;扫描电镜 中图分类号:白94.4 文献标识码:A 文章编号:1 ∞7 -9084(2011)03 -0242 -05 Research on poUen morphology and germination of Camellia oleifera Abel U Chun -lin1, 2 ,YAO Xiao - hua 1 • ,YANG Shui - ping2 ,REN Hua - dong2 ,CAO Yong - qing ( 1. Research lnstitute 0/ Subtropical Forestry ,CAF ， Fuya暗 3114∞， Chinaj 2. College 0/ Resource and Envirom旧时， Southwest University , Chongqing 400716 ,China ) Abstract: To study the evolutionary status of oil - tea Camellia in Theacea and micro dynamic pollination and fertilization of the Camellia oleifera Abel ,pollens of three Cαmellia oleifera Abel species we陀 scaned by electron mi- croscope and the germination and growth of pollen on stigma were observed in this study. The results showed 由at the shape of pollen was mainly prolate to subspherical from the equatorial view , the pollen grain size were average , the aperture was 3 - colporat and the exterior was similar to orange skin or brain veins , which indicated that oil - tea Camellia might be primitive groups in the Theacea.ηle pollens began to germinate O. 5h after pollination , and penetrated half way of the style 8h after pollination. They entered the ovarγafter 24h , and comple