七年制8apcandagpresentationnk.pptVIP

Introduction A variety of cell types specialized in the presentation of peptide-MHC to lymphocytes, causing either tolerance or immunity. Dendritic cells, monocytes/macrophages and B cells are professional APCs. I. Dendritic cells MHC class I/II molecules CD1a, CD83, BDCA2 (human) CD11c ,33D1, NLDC145 (mouse) Co-stimulatory molecules: B7.1(CD80)/B7.2(CD86), CD40, CD44, CD54 3. Classification of DC Lymphoid tissue DC follicular DC (FDC), interdigitating DC (IDC), thymic DC Non-lymphoid tissue DC Langerhans cell (skin), interstitial DC Circulating DC peripheral blood DC, veiled cell (lymph fluid) 4. Development of myeloid DC DC precursors Monocytes are the common precursor of macrophage and DC Immature DC Phenotype: high expression of receptors related to phagocytosis (FcR, CR, mannose receptor, DC-sign); low expression of CD54, CD40, CD80; CD86 and MHC IIhigh, CD14- Function: 1) strong capacity to ingest and process Ags, but weak ability to present Ags 2) induction of immune tolerance 3) sensing of infectious agents by TLR (pattern recognition receptors) Mature DC Phenotype: low expression of receptors related to phagocytosis (FcR, CR, mannose receptor); high expression of CD54, CD40, CD80, CD86 and MHC II; CD83+ and CD25+ Function: weak ability to capture and process MHC II presented Ags, powerful ability to present MHC I and II presented Ags. 5. DC and immune activation and immune tolerance 1) DC and immune activation DC present antigen and activate na?ve T cells DC may capture Ag by receptor-mediated endocytosis, macropinocytosis and phagocytosis; MHC II-Ag: CD4+ T cells MHC I-Ag: CD8+ T cells Lipid and glycolipid: presented by CD1 Immune regulation: Up-regulation: Ag-presentation;secretion of cytokines that up-regulate immune response,e.g.IL-12,IL-18 Down-regulation: inhibito