第5章2蛋白分选与膜泡运输31.pptVIP

The nuclear pore complex (NPC) 1 Spoke: Two spoke-rings: inner and outer Two rings: cytoplasmic ring and nuclearoplasmic ring 2.A nuclear basket 3. Filament : Eight cytoplasmic filament A lot proximal filaments 4. A Central transporter video 7 Figure 12-9a Molecular Biology of the Cell (? Garland Science 2008) The Arrangement of NPCs in the Nuclear Envelope Figure 12-9a Molecular Biology of the Cell (? Garland Science 2008) SEM of NPC from an Oocyte p148 Cytoplasmic face of the nuclear envelope showing the peripheral granules of the NPC Nuclear face of the nuclear envelope showing the basket-like appearance of the inner portion of the NPC. Figure 12-8 Molecular Biology of the Cell (? Garland Science 2008) Nuclear Pore Complexes Perforate the Nuclear Envelope The double-membrane envelope is penetrated by pores in which NPCs are positioned and is continuous with the ER. The nuclear lamina is a fibrous meshwork under- lying the inner membrane. Importing proteins from the cytoplasm to nucleus ◆ nuclear protein(核蛋白) ◆nuclear localization signals ,NLS(核定位信号) ◆nuclear export signals, NES(核输出信号) ◆ Central Transporter Transporter receptors ◆输入蛋白(importin) ◆输出蛋白(exportin) Figure 12-10 Molecular Biology of the Cell (? Garland Science 2008) A model for the gated diffusion barrier of the NPC Figure 12-11 Molecular Biology of the Cell (? Garland Science 2008) Nuclear Localization Signals (NLS) Direct Nuclear Proteins to the Nucleus The SV40 virus T-antigen contains the lysine-rich sequence and is imported into the nucleus. T-antigen with a Thr replacing Lys remains in the cytosol. NLS Importing Proteins from the Cytoplasm into the Nucleus 1. NLS-protein complexes with importin αβ 2. Binds with cyto- plasmic filament. 3. Moves through the nuclear pore. 4. Into the nucleoplasm, interacts with Ran-GTP dissociates. 5. Importin β associates with Ran-GTP, back to the cytoplasm, hydrolyzed. NLS Evidence for the Previous Model Gold particles covered